| 冬凌草多糖脱蛋白和脱色方法的研究 |
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| 引用本文： | 刘芳,朱学慧,刘玫,周晶,车宪平,韩瑞发.冬凌草多糖脱蛋白和脱色方法的研究[J].中药材,2008,31(5):751-753. |
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| 作者姓名： | 刘芳 朱学慧 刘玫 周晶 车宪平 韩瑞发 |
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| 作者单位： | 1. 天津医科大学药学院,天津,300070
2. 天津市泌尿外科研究所,天津,300211 |
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| 基金项目： | 天津医科大学校科研和教改项目 |
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| 摘 要： | 目的：筛选冬凌草多糖脱蛋白、脱色的最佳工艺。方法：以提取物多糖损失量、蛋白去除率和透光能力为测定指标,考察不同方法对冬凌草多糖脱蛋白、脱色的影响。结果：三氯乙酸脱蛋白法、活性炭脱色法为最佳工艺。结论：该除杂脱色方法为冬凌草多糖进一步研究提供了基础。
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| 关 键 词： | 冬凌草 多糖 脱蛋白 脱色 冬凌草 多糖 脱蛋白 脱色方法 研究 Polysaccharides Extraction Deproteinization 除杂 脱色法 活性炭 三氯乙酸 结果 影响 考察 测定指标 能力 透光 蛋白去除率 损失量 |
Study on Deproteinization and Decoloration in Extraction of Polysaccharides from Rabdosia rubescens |
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| LIU Fang,ZHU Xue-hui,LIU Mei,ZHOU jing,CHE Xian-ping,HAN Rui-fa.Study on Deproteinization and Decoloration in Extraction of Polysaccharides from Rabdosia rubescens[J].Jorunal of Chinese Medicinal Materials,2008,31(5):751-753. |
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| Authors: | LIU Fang ZHU Xue-hui LIU Mei ZHOU jing CHE Xian-ping HAN Rui-fa |
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| Institution: | College of Pharmacy, Tianjin Medical University, Tianjin 300070, China. |
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| Abstract: | OBJECTIVE: To screen the method on deproteinization and decoloration in extraction of of polysaccharides from Rabdosia rubescens. METHODS: The effect of deproteinization with different methods was evaluated in terms of the ratio of protein removing and polysaccharide removing, the decoloration effect was also calculated. RESULTS: The deproteinization effect was optimal with TCA method and activated carbon also achieved a high decoloration rate. CONCLUSION: The result can establish foundation for the further study of the polysaccharides from Rabdosia rubescens. |
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