益母草药材生物检定方法的研究(Ⅱ)——缩宫素、益母草量效关系和检定适用效应模式的建立

目的:探明益母草检定适用剂量范围,建立效应模式。方法:在同一标准子宫模型上,通过建立缩宫素、益母草各自的剂量效应曲线,构建适用于平行线四点法的效应模式。结果:子宫肌收缩效应在20～85mm范围内,益母草与缩宫素的对数剂量和反应高度呈线性关系,且基本平行。结论:在上述模式内,采用平行线四点法,以缩宫素作标准品,对益母草进行生物检定,同一样品三次检定结果平均可信限率均小于20％。

Study on the Biological Assay of Herba Leonuri -- Analysis the Dosage Response Curve of Herba Leonuri and Oxytocin and Establishment of Adequate Potency Pattern

Objective: To establish the effective pattern of Herba Leonuri to be suitable for biological assay. Methods: By comparing the dosage-effect curve of Herba Leonuri and oxytocin the suitable effective pattern was set up. Results:The experiment suggest that the uterus contract height from 20mm to 85mm is the adequate effective range. In this range, the effect both Herba Leonuri and oxytocin should plot as a straight line against the log dose and two lines are basically parallel. Conclusion: In the light of establishing effective pattern, the biological assay of Herba Leonuri may pattern the method of oxytocin biological assay to regard oxytocin as standard.The result error of Herba Leonuri assay is under 20% ,not exceed the demand limits of biological assay.