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| 白藜芦醇影响高转移卵巢癌细胞HO-8910PM转移相关能力的实验研究 | |
| 引用本文： | 覃燕梅,黎科,何太平,莫丽儿,梁念慈.白藜芦醇影响高转移卵巢癌细胞HO-8910PM转移相关能力的实验研究[J].中药材,2007,30(2):176-178. |
| 作者姓名： | 覃燕梅 黎科 何太平 莫丽儿 梁念慈 |
| 作者单位： | 1. 广东医学院生物化学与分子生物学研究所,广东湛江,524023 2. 深圳大学,广东深圳,518060 3. 广东天然药物研究与开发重点实验室,广东湛江,524023 |
| 基金项目： | 国家自然科学基金;湛江市"988"兴湛计划项目 |
| 摘 要： | 目的:研究白藜芦醇对人高转移卵巢癌细胞HO-8910PM转移相关能力的影响。方法:以MTT法检测白藜芦醇对高转移卵巢癌细胞HO-8910PM的细胞毒作用;用Transwell小室法检测白藜芦醇对HO-8910PM细胞侵袭能力和趋化运动能力的影响;以细胞粘附人工重组基底膜实验检测白藜芦醇对HO-8910PM细胞粘附能力的影响。结果:白藜芦醇作用细胞6 h后能抑制HO-8910PM细胞体外趋化运动和粘附能力,其中100μmol/L抑制率分别为(30.1±10.8)%,(34.27±1.28)%,但白藜芦醇并不影响HO-8910PM细胞侵袭人工基底膜能力。结论:白藜芦醇能抑制高转移卵巢癌细胞HO-8910PM的运动和粘附能力,是潜在的抗肿瘤转移药物。 |
| 关 键 词： | 白藜芦醇 侵袭 运动 粘附 转移 卵巢癌 |
| 文章编号： | 1001-4454(2007)02-0176-03 |
| 修稿时间： | 2006-07-05 |
Study on the Effect of Resveratrol on Metastasis-associated Ability of Ovarian Carcinoma HO-8910PM Cells in Vitro |
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| QIN Yan-mei,LI Ke,HE Tai-ping,MO Li-er,LIANG Nian-ci.Study on the Effect of Resveratrol on Metastasis-associated Ability of Ovarian Carcinoma HO-8910PM Cells in Vitro[J].Jorunal of Chinese Medicinal Materials,2007,30(2):176-178. | |
| Authors: | QIN Yan-mei LI Ke HE Tai-ping MO Li-er LIANG Nian-ci |
| Institution: | 1. Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China; 2. Shenzhen University, Shenzhen 518060, China; 3. Guangdong Key Laboratory for Research and Development of Natural Drugs, Zhangjiang 524023, China |
| Abstract: | OBJECTIVE: To investigate the inhibitory effect of resveratrol on the metastasis-associated ability of human highly metastatic ovarian carcinoma HO-8910PM cells in vitro. METHODS: MTf assay was used to examine the cytotoxicity of resveratrol in HO-8910PM cells; Transwell Chamber assay was performed to determine the effect on invasion and migratory capacity of the cells by resveratrol; Effect on adhesion potential of HO-8910PM cells was tested by cell-Matrigel adhesion assay. RESULTS: Resveratrol showed no cytotoxicity on HO-8910PM cells after 6 h treatment. Resveratrol significantly inhibited migration and adhesion capacity of HO-8910PM cells in vitro. Their inhibitory rates after treated with the chemical of 100 micromol/L for 6 h were (30. 1 +/- 10. 8) % ,(34. 27 +/- 1. 28)% , respectively. However, Resveratrol had no effect on invasion capacity of HO-8910PM cells. CONCLUSION: Resveratrol can inhibit the migration and adhesion of HO-8910PM cells in vitro. Resveratrol might be a potential drug to inhibit tumor metastasis. |
| Keywords: | Resveratrol Invasion Migration Adhesion Metastasis Ovarian carcinoma |
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