| 几种金丝桃属药用植物的金丝桃苷含量测定 |
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| 引用本文： | 万德光,裴瑾,严铸云.几种金丝桃属药用植物的金丝桃苷含量测定[J].中药材,2004,27(6):397-398. |
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| 作者姓名： | 万德光 裴瑾 严铸云 |
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| 作者单位： | 成都中医药大学药学院,成都,610075 |
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| 摘 要： | 目的:比较金丝桃属药用植物中金丝桃苷含量,为筛选新的药用品种以及为定量评价提供参考依据.方法:采用HPLC法测定金丝桃属3组5种1亚种金丝桃苷的含量.结果:金丝桃苷的进样量在0.101～2.023μg范围内峰面积值与进样量之间线性关系良好,平均回收率为96.527%.贯叶连翘的含量最高1.257%,其次是川滇金丝桃0.461%,含量最低是元宝草0.137%.结论:本方法的稳定性和重现性较好,可作为药材的品质评价方法之一.贯叶连翘、川滇金丝桃等金丝桃属药用植物有进一步研究、开发价值.
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| 关 键 词： | 金丝桃属 金丝桃苷 |
| 修稿时间： | 2003年9月15日 |
Determination of Hyperin in Genus Hypericum Medicinal Plants |
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| Wan Deguang,Pei Jin,Yan Zhuyun.Determination of Hyperin in Genus Hypericum Medicinal Plants[J].Jorunal of Chinese Medicinal Materials,2004,27(6):397-398. |
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| Authors: | Wan Deguang Pei Jin Yan Zhuyun |
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| Institution: | College of Pharmacy, Chengdu University of TCM, Chengdu 610075. |
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| Abstract: | OBJECTIVE: To compare the content of hyperin in Hypericum medicinal plants. METHODS: HPLC method was established. Alltima C18 radial compression column was used with a mobile phase of methanol-0.5% phosphoric acid (45:55, adjusted to pH = 3.0 with triethylamine). The detecting wavelength was 360 nm. Flow rate was 0.8 ml/min, external standard method was quantitative analysis method. RESULTS: The linear range was 0.101-2.023 microg. The average recovery was 96.527%. The result showed that the content of hyperin in H. perforatum, H. forrestii was higher than that of others (1.257% and 0.461% respectively). CONCLUSION: This method provides an accurate and sensitive way in detecting hyperin. |
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| Keywords: | HPLC |
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