黄连解毒汤有效部位对实验性脑缺血的保护作用

目的:观察黄连解毒汤有效部位(HLJDTAF)对实验性脑缺血的保护作用.方法:采用三氯化铁所致大鼠单侧大脑中动脉阻塞(MCAO)模型观察HLJDTAF对局灶性脑缺血的保护作用;采用大鼠双侧颈总动脉结扎(CCAO)模型研究药物对脑毛细血管通透性的影响;采用小鼠双侧颈总动脉反复缺血再灌注(CCAORR)模型观察药物对模型断头张口喘气时间、脑指数与脑含水量的影响.结果:HLJDTAF可明显减轻MCAO大鼠神经症状,减少脑梗塞范围(P＜0.05,P＜0.01);HLJDTAF还能显著降低CCAO大鼠血脑屏障毛细血管通透性及脑组织EB含量(P＜0.05,P＜0.01);该有效部位还可以延长CCAORR的断头张口喘气时间,降低脑指数,减轻脑水肿(P＜0.05,P＜0.01).结论:HLJDTAF具有很好的脑保护作用.

The Effect of "Huang Lian Jie Du Tang" Active Fraction on Experimental Cerebral Ischemia

Objective:To study the protective effect of "Huang Lian Jie Du Tang" active fraction(HLJDTAF) on experimental cerebral ischemia. Methods:Middle cerebral artery occlusion(MCAO) in rats was used to observe the protective effect of HLJDTAF on ischemia stroke. Common carotid artery occlusion(CCAO) rat was used to analyse the effect of HLJDTAF on blood brain barrier (BBB) permeability. Common carotid artery occlusion and reperfusion repeatedly(CCAORR) in mice were used to test effect of HLJDTAF on gasp time,brain exponential and water content of brain. Results: HLJDTAF could improve the abnormal behavior of MCAO rats and decrease the infarction area (P<0.05, P<0.01). HLJDTAF could also significantly prolong the shorten gasp time of CCAORR mice,reduce the brain exponential and decrease the water content in brain (P<0.05, P<0.01). Conclusions: HLJDTAF can protect the brain ischemia injury.