化湿液对湿阻证大鼠胃粘膜损伤修复的影响

目的:研究化湿液对湿阻证模型大鼠胃粘膜损伤修复的影响。方法:将50只SD大鼠随机分为正常组,模型组,化湿液低、中、高剂量组,每组10只。除正常组外,其余4组用改进后的环境加疲劳法制造湿阻证模型,连续造模6d。造模成功后,正常组和模型组大鼠按2 ml/100 g灌胃给予生理盐水;化湿液低、中、高剂量组分别以0.4、0.8、1.6 g生药/100 g灌胃给予化湿液,1次/d,连续8 d后观察各组大鼠胃粘膜损伤情况;取大鼠胃粘膜组织,检测SOD、MDA和NO含量。结果:与正常组比较,模型组大鼠胃粘膜出现充血、水肿,并伴有大量出血点或凝血块,胃粘膜SOD和NO含量显著降低(P<0.05),MDA含量显著增加(P<0.05);与模型组比较,化湿液治疗后湿阻证大鼠胃粘膜充血、水肿和出血等症状完全消失,同时胃粘膜SOD及NO含量增加(P<0.05),MDA含量降低(P<0.05)。结论:化湿液对湿阻证大鼠胃粘膜损伤具有修复作用,其机制可能与提高胃粘膜SOD及NO含量,降低胃粘膜MDA含量有关。

Effects of Huashi Liquid on the Repair to Impaired Gastric Mucosa in Rats with Dampness Retention Syndrome

Objective: To investigate the effects of Huashi Liquid on the repair to impaired gastric mucosa in rats with dampness retention syndrome.Methods:Fifty SD rats were randomly divided into normal group,model group,high,middle and low-dose of Huashi Liquid groups,10 rats in each group.Dampness retention syndrome was induced in all rats,except the normal group,by modified enviromental and fatigued method for 6 days.After the model was formed,the normal and model groups were fed with saline(NS) in 2 ml/100 g dose,high,middle and low-dose of Huashi Liquid groups were fed with Huashi Liquid in different dose(0.4 g/100g,0.8 g/100 g,1.6 g/100 g) respectively,one time per day,last for 8 days.Then,the impaired extent of gastric mucosa was observed and the content of superoxide dismutase(SOD),malondialdehyde(MAD) and nitric oxide(NO) in gastric mucosa ware detected.Results:In gastric mucosa of model group,Hyperemia,edema and massive hemorrhagic spots or sludged blood existed,SOD and NO significantly decreased(P<0.05),MAD significantly increased(P<0.05),compared with the normal group.In gastric mucosa of all the Huashi Liquid,symptoms such as Hyperemia,edema and hemorrhage completely disappeared,SOD and NO significantly increased(P<0.05),MDA significantly decreased(P<0.05),compared with the model group.Conclusion:Huashi Liquid could repair gastric mucosa impairment of rats with dampness retention,the mechanism may be related to increasing SOD,MAD and decreasing NO content in gastric mucosa.