博落回总生物碱分离纯化工艺研究

本研究以博落回总生物碱含量及回收率等为考察指标，研究大孔吸附树脂分离纯化博落回总生物碱工艺。结果表明：AB．8型大孔吸附树脂对博落回总生物碱静态饱和吸附量为104．65mg／g（干树脂），洗脱率95．9％，动态饱和吸附量为96．5mg／g（干树脂），总生物碱回收率在91．24％、纯度在90％以上，是实验树脂中分离纯化博落回总生物碱的最佳大孔吸附树脂。分离纯化博落回总生物碱最佳工艺条件为：AB-8型大孔吸附树脂，洗脱剂为90％乙醇，洗脱剂用量为2-3倍树脂体积，上柱总生物碱量与树脂比为1：10．4，上柱液总生物碱浓度为21．57mg／ml，流速2-3ml／min，上柱液pH值7—8。

Study on Separation and Purification of Total Alkaloids from Macleaya cordata

A method for separation and purification of total alkaloids from Macleaya cordata with macroporous resin was studied, with the content and recovery rate of total alkaloids as indexes. The results were as follows: The static adsorption capacity of AB-8 type resin was 104. 65 mg/g, the static elution ratio were 95.9% , the dynamic adsorption capacity of AB-8 type resin was 96.5 mg/g, the recovery rate was more than 91.24% and the purity was more than 90%. AB-8 type resin was the best for separating and purificating Macleaya cordata in total alkaloids. The optimum conditions is: the eluant is 90% alcohol and 2-3 times as the volume of the resin, the volume of the resin is 10.4 times of total alkaloids in sample, concentration of total alkaloids of sample is 21.57 mg/ml and current velocity of 2-3 ml/min, pH value of sample is 7-8.