复方六月雪对鸭乙型肝炎病毒DNA的抑制作用

目的:观察复方六月雪(CLYX)对鸭乙型肝炎病毒(DHBV)DNA的抑制作用,为研究和开发新的抗HBV药物提供实验依据。方法:采用1日龄雏鸭接种广西麻鸭DHBV强阳性血清,13天后用PCR法筛选出DHBV强阳性鸭。随机分成5组:CLYX高、中、低剂量组、模型组和阳性对照组,每组10只,各组雏鸭均灌胃给药14天。于用药前(T0)、用药7天(T7)、14天(T14)及停药后3天(P3)分别采血,采用荧光定量PCR(FQ-PCR)法检测血清DHBVDNA的含量。结果:CLYX各剂量组用药后血清DHBV DNA含量显著降低(P<0.01),停药3天后,CLYX低剂量组和阳性对照组血清DHBV DNA有反跳现象,而CLYX中、高剂量组血清DHBV DNA没有反跳现象。CLYX抑制DHBV DNA的作用有明显的量效和时效反应关系。结论:CLYX可有效地抑制DHBV DNA的复制。

The Inhibitory Effect of Compound Liuyuexue on DHBV DNA

Objective: To study the inhibitory effect of traditional Chinese medicine Compound Liuyuxeue(CLYX) on duck hepatitis B virus(DHBV) DNA,and provide experimental basis for developing a new drug for the clinical treatment.Methods: One-day old Guangxi brown spotted ducks infected with DHBV were used as the hepatitis B virus infected animal model.Positive ducks were detected by PCR at 13 days after the infection of DHBV,and were randomly divided into five groups: the high dose group,middle dose group and low dose group of Compound Liuyexue(CLYX),model group,positive control group.Every group had 10 ducks and CLYX was given for 14 days.The content of DHBV DNA in serum were measured by Fluoresceence Quantitative PCR(FQ-PCR).Results: The serum DHBV DNA content was decreased significantly by the treatment with CLYX.The high dose group and middle dose group of CLYX could significantly inhibit DHBV DNA replication in vivo(P<0.01).DHBV DNA content in serum in high dose group and middle dose group did not return significantly 3 days after stopping treatment,and its inhibitory effects were dose-and tim-dependents.Conclusion: CLYX can inhibit significantly DHBV DNA.