| HPLC法测定宁神静心片中天麻素的含量 |
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| 引用本文： | 强小娟,文飞燕,骆勇.HPLC法测定宁神静心片中天麻素的含量[J].湖南中医药导报,2013(12):99-101. |
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| 作者姓名： | 强小娟 文飞燕 骆勇 |
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| 作者单位： | [1]彭州市中医医院,四川彭州611930 [2]四川大学华西药学院,四川成都610041 |
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| 摘 要： | 目的：建立宁神静心片中天麻素的含量测定方法.方法：采用HPLC法,以Kromasil C18柱（250 mm×4.6 mm,5μm）为色谱柱;以乙腈为流动相A,以0.05％磷酸溶液为流动相B,进行梯度洗脱;流速：1 mL·min-1;柱温：25℃;检测波长：220 nm.结果：天麻素进样量在15-100 μg· ml-1,A=15357C＋34136,范围内线性关系良好,r=0.9998;天麻素的平均加样回收率为98.42％,RSD％为2.86％（n=6）.结论：所建立的方法准确、专属性强、重复性好,可有效控制宁神静心片的质量.
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| 关 键 词： | 宁神静心片 天麻素 HPLC法 |
HPLC Determination of Gastrodin in Ning-shen Jing-xin Tablet |
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| QIANG Xiao-juan,WEN Fei-yan,LUO Yong.HPLC Determination of Gastrodin in Ning-shen Jing-xin Tablet[J].Hunan Guiding Journal of Traditional Chinese Medicine and Pharmacology,2013(12):99-101. |
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| Authors: | QIANG Xiao-juan WEN Fei-yan LUO Yong |
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| Institution: | 1.Pengzhou Hospital of Chinese Medicine, Pengzhou Sichuan 611930; 2.West China School of Pharmacy, Sichuan University, Chengdu Sichuan 610041;) |
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| Abstract: | Objective：To establish a HPLC method for determination of Gastrodin in Ning-shen Jing-xin n tablet.Methods：HPLC method was applied.The analytical column was a Kromasil C1s （250 mm×4.6 mm,5 μm）.The mobile phase consisted of acetonitrile（A） and 0.05％ Phosphoric acid gradient elute.The flow rate was 1.0 ml ·min-1 and the column temperature was 25 ℃.The determination wavelength was 220 nm.Results：The calibration curve was linear in the range of 15-100 μg·mL-1.The regression equation was A=15357C＋34136,r=0.9998.The average recovery was 98.42％,RSD％ was 2.86 ％ （n=6）.Conclusion：The method is accuracy,specific and reproducible for quality control of Ning-shen Jing-xin n tablet. |
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| Keywords: | Ning-shen Jing-xin tablet Gastrodin HPLC |
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