| 乙酰葛根素对大鼠脑缺血再灌注VEGF表达的影响 |
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| 引用本文： | 王坤芳,刘必旺,梁志刚,刘亚明.乙酰葛根素对大鼠脑缺血再灌注VEGF表达的影响[J].光明中医,2011,26(6):1122-1124. |
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| 作者姓名： | 王坤芳 刘必旺 梁志刚 刘亚明 |
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| 作者单位： | 王坤芳,刘必旺,刘亚明,Wang Kunfang,Liu Biwang,Liu Yaming(山西中医学院,太原,030024);梁志刚,Liang Zhigang(山西中医学院第二中医院,太原,030024) |
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| 基金项目： | 国家科技部"十二五"科技支撑项目，山西省国际科技合作项目 |
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| 摘 要： | 目的探讨脑缺血再灌注后乙酰葛根素的脑保护作用及其机制。方法 线栓法制作脑缺血再灌注模型,分别对假手术组、脑缺血再灌注组、葛根素组、乙酰葛根素低（10mg·kg-1）、中（50mg·kg-1）、高剂量（250mg·kg-1）干预组实验标本作HE染色,采用免疫组化方法观察测定各组大鼠脑内的VEGF的表达情况并进行神经功能缺损评分。结果 脑缺血再灌注组与假手术组比较,VEGF表达增多（P〈0.01）,药物干预组较缺血再灌注组VEGF表达均增多（P〈0.01）,且神经元损伤较轻,且三个剂量的表达有显著差别（P〈0.01）。结论 乙酰葛根素对脑缺血再灌注有明显的保护作用,诱导VEGF表达可能是其对脑缺血再灌注损伤起保护作用的机制之一。
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| 关 键 词： | 乙酰葛根素 脑缺血再灌注 VEGF 大鼠 |
The Effect of Acetylpuerarin on Apoptosis of Neuronal Cell and on the Expression of VGEF after Cerebral Ischemia and Reperfusion Rats |
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| Wang Kunfang,Liu Biwang,Liang Zhigang,Liu Yaming.The Effect of Acetylpuerarin on Apoptosis of Neuronal Cell and on the Expression of VGEF after Cerebral Ischemia and Reperfusion Rats[J].Journal of Traditional Chinese Medicine,2011,26(6):1122-1124. |
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| Authors: | Wang Kunfang Liu Biwang Liang Zhigang Liu Yaming |
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| Institution: | Wang Kunfang1 Liu Biwang1 Liang Zhigang2 Liu Yaming1 (1. Shanxi College of TCM,Shanxi Taiyuan 030024,2. The Second Hospital of Shanxi College of TCM,Shanxi Taiyuan 030024) |
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| Abstract: | Objective To investigate the protective effect of acetylpuerarin on cerebral ischemia-reperfusion injury in rats and itsmechanism. Methods Wistar rat was used as a model of focal cerebral ischemia and reperfusion to observe expression of VEGF after focal cerebral ischemia-reperfusion and the protective function of three levels of doses Acetylpuerarin. Immunohistochemicalmethod were used to measure the number of VEGF positive cells in rat’s cerebral cortex of sham-operated group,model group and Acetylpuerarin group. Results VEGF had a high-level expression and increased significantly after ischemia reperfusion(P<0.01).The Acetylpuerarin can significantly increase the expression of the VEGF,and the highest expression after the highest Acetylpuerarin group (P<0.01).Conclusion The Acetylpuerarin had obviously protective function for ischemia-reperfusion rat. |
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| Keywords: | VEGF |
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