扶正消癌方对乳腺癌患者癌细胞增殖、侵袭及凋亡的影响

目的:探讨扶正消癌方对乳腺癌患者癌细胞增殖、侵袭和凋亡的影响,并研究凋亡分子机制。方法:66例乳腺癌患者随机分为对照组30例和治疗组36例,对照组患者接受化疗药物表阿霉素和环磷酰胺治疗,治疗组患者在对照组基础上加用扶正消癌方,日1剂,连服9周;2组患者治疗结束后接受手术,术中留取瘤体组织。用免疫组化法检测乳腺癌组织中Ki67、MMP2、Fas基因的表达水平,Western blotting分析凋亡分子Bax、Bcl-2的蛋白表达情况。结果:治疗组乳腺癌组织Fas阳性率明显高于对照组(P0.05);治疗组乳腺癌组织Ki67、MMP2的阳性表达率明显低于对照组;Western blotting结果显示治疗组乳腺癌细胞Bax蛋白表达显著多于对照组,而Bcl-2蛋白表达明显少于对照组,差异比较均有统计学意义(P0.05)。结论:扶正消癌方可抑制乳腺癌细胞的增殖和侵袭转移,促进癌细胞凋亡,这可能是扶正消癌方治疗乳腺癌的作用机制。

Effects of Fuzheng Xiaoai Decoction on Cell Proliferation, Invasion and Apoptosis in Tissues of Breast Cancer

To investigate the effects of Fuzheng Xiaoai Decoction on cell proliferation, invasion and apoptosis in tissues of breast cancer, and its relevant apoptotic molecular mechanisms. Methods:A total of 66 cases of breast cancer were randomly divided into the control group (30 cases) and the treatment group (36 cases). The control group received chemotherapy drugs doxorubicin and cyclophosphamide. On the basis of the control group, the treatment group were treated with Fuzheng Xiaoai Decoction once a day for nine weeks. Ki67, MMP2 and Fas protein expression levels were determined by immunohistochemical technology. The protein levels of Bax, Bcl-2 were detected by western blotting. Results:The Fas expressions of breast cancer tissues were significantly higher in treatment group than the control group (P<0.05). The Ki67, MMP2 expressions in breast cancer tissues were lower in treatment group compared with the control group (P<0.05). Results of western blotting indicated that the protein level of Bax in breast cancer tissues of the treatment group increased significantly compared with the control group, but the Bcl-2 expression level decreased significantly, the differences were statistically significant (both P<0.05). Conclusion:Fuzheng Xiaoai Decoction can inhibit the proliferation and invasion breast cancer cells, promote the apoptosis of breast cancer cells, which may be the molecular mechanism of Fuzheng Xiaoai Decoction in the treatment of breast cancer.