基于高通量测序技术研究药食两用薏苡仁中污染真菌多样性

目的:调查药食两用薏苡仁中污染真菌多样性,为其安全使用提供参考依据。方法:收集薏苡仁样品18批,提取真菌DNA并扩增ITS2序列,基于Illumina MiSeq PE250平台进行高通量测序。结果:共检测到4门18纲44目99科149属的真菌,子囊菌门Ascomycota是最优势菌门,镰刀菌属Fusarium(3.05%~60.32%)是属水平最优势属,其次是曲霉属Aspergillus(2.20%~45.44%)、白僵菌属Beauveria(0.07%~63.21%)、链格孢属Alternaria(0.80%~11.92%)、Arachnomyces(0.03%~39.36%)和青霉属Penicillium(0.24%~8.03%)。此外,共检测到5种潜在产毒真菌,分别是烟曲霉A.fumigatus、土曲霉A.terreus、梨孢镰刀菌F.poae、囊状青霉P.capsulatum和展青霉P.paxilli。结论:高通量测序技术可以快速有效地检测薏苡仁中污染真菌种类,为薏苡仁污染真菌毒素提供风险预警。

Analyzing Fungal Diversity in Medicinal and Edible Coicis Semen using High-throughput Sequencing

Objective:To investigate the fungal diversity in medicinal and edible Coicis Semen,thus providing evidence for its safe use.Methods:A total of 18 batches of Coicis Semen samples were collected.The total DNA was extracted,and the ITS2 sequences were amplified.Then,the amplicons were sequenced using the Illumina MiSeq PE250 platform.Results:The fungi detected were denominated to 4 phylum,18 class,44 orders,99 families,and 149 genera.Ascomycota was the dominant fungus at the phylum level.At the genus level,Fusarium was the most predominant,followed by Aspergillus,Beauveria,Alternaria,Arachnomyces and Penicillium,accounting for(3.05%~60.32%),(2.20%~45.44%),(0.07%~63.21%),(0.80%~11.92%),(0.03%~39.36%) and(0.24%~8.03%).In addition,a total of 5 potential mycotoxin-producing fungi were detected,namely,A.fumigatus,A.terreus,F.poae,P.capsulatum and P.paxillin.Conclusion:High-throughput sequencing technology can quickly and effectively detect fungal contamination in Coicis Semen,thereby providing early warning for mycotoxin contamination in Coicis Semen.