经穴注射BMSCs联合中药对下肢缺血大鼠促血管生成相关因子的影响

目的:观察经穴注射骨髓间充质干细胞(Bone Marrow Mesenchymal Stem Cells,BMSCs)联合益气活血中药对糖尿病(Diabetes Mellitus,DM)大鼠缺血后肢骨骼肌中促血管生成因子的表达情况。方法:80只SD大鼠1∶7随机分为2组:正常假手术组10只,其余70只一次性腹腔注射链脲佐菌素(Streptozotocin,STZ,50.0 mg/kg)制成DM大鼠模型,再等比例随机分为7组,即DM假手术组、DM缺血组、益气活血中药组、局部注射BMSCs组、局部注射BMSCs+中药组、穴位注射BMSCs组、穴位注射BMSCs+中药组,每组10只。应用RT-PCR法检测大鼠后肢骨骼肌中VEGF、b FGF mRNA的表达。结果:穴位注射BMSCs+中药组术侧VEGF mRNA表达量较DM假手术组升高(P0.05);DM假手术组、局部注射组、穴位注射组术侧b FGF mRNA表达量较健侧升高(P0.05);局部注射+中药组、穴位注射+中药组术侧b FGF mRNA表达量较正常血糖假手术组、DM假手术组、DM缺血组升高(P0.05);穴位注射+中药组健侧b FGF mRNA表达量较正常血糖假手术组、DM假手术组、DM缺血组、中药组、局部注射组升高(P0.05)。结论:穴位注射BMSCs结合益气活血中药可明显促进血管生成因子VEGF、b FGF的表达,益气活血中药可能促进穴位注射BMSCs的成活率从而提高促血管生成因子VEGF、b FGF的表达。

The Effect of Acupoint Injection of BMSCs Combined wih Chinese Medicine on VEGF/bFGF of Diabetic Rats with Lower Limbs Ischemic

To observe the expression of angiogenesis factor in skeletal muscle of diabetic rats with lower limbs ischemia during acupoint injection of bone marrow mesenchymal stem cells (BMSCs) with Qi-tonifying and Blood-activating Chinese medicine. Methods: Eighty Sprague Dawley (SD) rats were randomly divided into 2 groups, 10 rats in the normal blood sugar control (NMC) grooup. The other 70 rats were intraperitoneally injected Streptozotocin (50.0 mg/kg) to make DM model, then were randomly divided into 7 groups equally, namely diabetes control (DMC) group, diabetes ischemic (DMI) group, Qi-tonifying and Blood-activating (QB) group, local injection BMSCs (L-BMSCs) group, L-BMSCs + QB group, acupoint injection BMSCS (Acu-BMSCs) group, and Acu-BMSCs + QB group. Using RT-PCR method to detect the expression of VEGF and bFGF mRNA in muscular tissue. Results: The expression of VEGF mRNA on the operation side in Acu-BMSCs + QB group was higher than DMC group (P<0.05). The expression of bFGF mRNA on the operation side in DMC, L-BMSCs, Acu-BMSCs groups were higher than the uninjured side(P<0.05), and those in L-BMSCs, Acu-BMSCs + QB groups were higher than NMC, DMC, DMI groups(P<0.05). The expression of bFGF mRNA on the uninjured side in Acu-BMSCs + QB group was higher than NMC, DMC, DMI, QB, L-BMSCs groups(P<0.05). Conclusion: The expression of VEGF, bFGF were positively affected by acupoint injection of BMSCs combined with QB, which means QB Chinese herb could improve the expression of angiogenesis factor by increasing the survival rate of BMSCs injected in acupoint.