颐脑解郁方对缺血性脑卒中及卒中后抑郁、焦虑、痴呆大鼠宏观表征变化的干预作用

目的:探究缺血性脑卒中大鼠向卒中后抑郁、焦虑、痴呆演变过程中宏观表征动态变化情况以及中药颐脑解郁方的干预作用。方法:选取雄性wistar大鼠100只,随机分为卒中模型组、卒中观察组、卒中假手术组、PSD模型组、PSD观察组、PSD假手术组、PSA模型组、PSA观察组、PSA假手术组、VaD模型组、VaD观察组、正常组。除正常组及假手术组外,均进行MCAO手术建立缺血性脑卒中模型,术后分别复合CUMS建立PSD模型、复合不确定性空瓶饮水刺激建立PSA模型,VaD模型为术后正常喂养28 d后通过水迷宫实验筛选而出。卒中组术后药物干预2周,其余各组应激结束后药物干预4周。观察组灌胃颐脑解郁方,余各组灌胃蒸馏水。连续采集各组大鼠宏观表征信息,并比较卒中组治疗1、2周及PSD、PSA、VaD组治疗1、2、4周体重变化情况。结果:卒中大鼠向PSD演变过程中,以先出现易激惹表现,而后活动度、精神状态、反应性逐渐下降,口鼻黯淡,舌色紫暗,舌下脉络发绀为特征;卒中大鼠向PSA演变过程中表征变化为攻击性、探究性增加,梳理行为减少,口鼻黯淡,舌红少津等;向Va D演化过程中,具有活动度、精神状态、反应性逐渐下降趋势,口鼻色稍暗,舌润泽,舌色淡红。各模型大鼠体重均较正常组明显下降。应用颐脑解郁方干预后,观察组大鼠宏观表征改善,体重显著增加。结论:PSD、PSA、VaD在其演变过程中具有肾虚肝郁的共同病机,益肾调气方药颐脑解郁方对缺血性脑卒中及卒中后精神疾病具有一定疗效。

Effects of Yinao Jieyu Decoction on Macroscopic Characteristics in Rat Models of Ischemic Stroke and Post-stroke Depression,Anxiety and Vascular Dementia

To investigate the dynamic changes of macroscopic characteristics in the development and evolution from ischemic stroke to post-stroke depression,anxiety and vascular dementia and the intervention effect of Yinao Jieyu Decoction.Methods:Male Wistar rats were randomly divided into ischemic stroke (IS) model group,IS therapy group,IS sham-operation group,post-stroke depression (PSD) model group,PSD therapy group,PSD sham-operation group,post-stroke anxiety (PSA) model group,PSA therapy group,PSA sham-operation group,vascular dementia (VaD) model group,VaD therapy group and normal group.Middle cerebral artery occlusion (MCAO) was operated in rats except those in normal group and sham-operation groups.After the operation for 7 days,PSD groups were given chronic unpredictable mild stress (CUMS) and isolation rearing for 21 days for duplicating PSD model,the PSA groups were accompanied by empty water bottle stimulation for 21 days for duplicating PSA model.After the operation for 28 days,the VaD groups were selected by Morris water maze,then put into the test.The IS groups were gavaged for 2 weeks after the operation,the other groups were gavaged for 4 weeks after modeling.Therapy groups were given Yinao Jieyu Decoction while model groups and sham-operation groups were given distilled water.Rats macroscopic characteristics were dynamically collected and the weight changes of rats in IS groups at the first and second weeks,PSD,PSA,and VaD groups at the first,second and forth weeks were compared.Results:In the evolution form stroke to PSD,rats showed irritability first,then the activity,mental state and reactivity gradually decreased.PSD rats represented dim lips and nose,dark purple tongue and purple sublingual vein.In the evolution from stroke to PSA,aggressive and explorative behaviors were increased,grooming behaviors were decreased.PSA rats represented dim lips and nose,red tongue with scant liquid.In the evolution from stroke to VaD,rats showed progressive decline in activity,mental status and responsiveness.VaD rats represented light lip color,moistened tongue and pink tongue.The weight of rats in model groups was significantly lower than that in the normal group.After the intervention of Yinao Jieyu Decoction,the macroscopic characteristics of rats were improved in therapy groups,and the body weight was increased significantly.Conclusion:PSD,PSA and VaD have a common pathogenesis of kidney deficiency and liver turbidity in the course of their evolution,Yinao Jieyu Decoction with the actions of replenishing kidney and regulating qi has a certain effect on ischemic stroke and mental disorders after stroke.