金钗石斛多糖调控炎性小体N LRP3对非酒精性脂肪肝大鼠的保护作用

目的:研究金钗石斛多糖对NAFLD大鼠的干预作用,并从NLRP3信号通路分析作用机制.方法:将72只SD大鼠分为正常、模型、金钗石斛多糖高、中、低剂量和多烯磷脂酰胆碱干预组,除正常组外,NAFLD模型构建成功治疗4周后处死,取出肝脏检测肝脏病理学和NLRP3、Caspase-1蛋白及mRNA表达,收集血液检测谷草转氨酶...

Protective Effects of Dendrobium Nobile Polysaccharide on NLRP3 in Rats with Nonalcoholic Fatty Liver Disease

Objective:To investigate the intervention effect of Dendrobium nobile polysaccharides on NAFLD rats,and to analyze the mechanism of action from the NLRP3 signaling pathway.Methods:A total of 72 SD rats were divided into 6 groups,which were the normal,model,high dose,medium dose,low dose Dendrobium nobile polysaccharide,and the polyene phosphatidylcholine intervention group.Except for the normal group,the other 5 groups were constructed an animal model of NAFLD and after treatment,the rats were sacrificed.Their liver was taken out to detect liver pathology and NLRP3,Caspase-1 protein and mRNA expression.Their blood was also collected to detect the AST,ALT,TG,TC,LDL-C,HDL-C,IL-1βand IL-18.Results:The liver lobules in the normal group were clear,and the nuclei were evenly distributed.The model group had obvious fatty vacuoles and inflammatory reaction infiltration.The Dendrobium nobile polysaccharide and polyene phosphatidylcholine group improved significantly.Compared with the control group,AST,ALT,TG,TC,HDL-C,LDL-C,IL-1β,IL-18,Caspase-1 and NLRP3 protein and mRNA were significantly increased in the model group(P<0.05).Compared with the model group,those indexes in the Dendrobium nobile polysaccharide and the polyene phosphatidylcholine group were significantly lower(P<0.05).In addition,there was no difference in AST,ALT and HDL-C between the Dendrobium nobile polysaccharide group and the polyene phosphatidylcholine group.TG,TC and LDL-C of the high-dose group were lower than those of the polyene phosphatidylcholine group(P<0.05).The protein and mRNA of IL-1β,IL-18,Caspase-1 and NLRP3 in the high-dose group were significantly lower than those in the polyene phosphatidylcholine group(P<0.05).Conclusion:Dendrobium nobile polysaccharides can significantly improve the liver function and blood lipids of NAFLD rats induced by a high-fat and high-sugar diet,which is related to its ability to regulate the inflammasome NLRP3,thereby reducing the inflammatory response.