小型猪痰瘀互结证冠心病证侯诊断研究

目的：研究小型猪痰瘀互结证冠心病中医证侯诊断指标及方法。方法：小型猪随机分为正常对照组和模型对照组,分别用普通饲料和高脂饲料喂养14周,2周时采用介入技术球囊损伤左冠状动脉前降支,制备小型猪冠心病模型。测定30点体表心电图、体重指数、采集舌象以及无创血流动力学指标,采集股动脉脉搏图像,分别对胸痹心痛的主症、兼症、舌象及舌下脉络和脉象进行标准化评分,分析舌象RGB及HIS数值变化,对脉搏图进行分析。结果：与正常对照组比较,模型对照组动物的主症、兼症、舌象和脉象均有明显的改变,此评价方法具有较好的稳定性;模型组舌象图像R分量下降,红色饱和度降低;模型组动物在实验2周时表现出脉搏图h3/h1改变。结论：证候客观化评分方法在实验小型猪冠心病痰瘀互结证研究中有较好的稳定性,规范化的舌象图像采集有助于评价疾病变化,而脉搏图评价复合因素造模实验动物的脉象的方法还需探索。

Research on Symptoms for Coronary Heart Disease Model of Mini swine with Phlegm-stasis Syndrome Type

Objective： To set up diagnosis methods of mini swine coronary heart disease（ CHD） angina model of phlegm-stasis syndrome type. Methods： Mini swine were randomly divided into the control group and the model group,fed with common forage and high fat forage respectively for 14 weeks and the coronary left anterior descending branch in the model group was injured by balloon intervention technique after 2-week feeding to establish CHD model. The model establishment and its physiopathology indices were evaluated by examinations on body surface electrocardiograph（ BS-ECG）,body mass index（ BMI）,pulse conditions,tongue pictures,noninvasive heart function monitor,arteria femoralis pulse wave. Using ECG as the principal index,BMI as secondary index,tongue picture as tongue manifestation,and arteria femoralis pulse wave as pulse manifestation,giving scores based on above criteria. Results： Principal symptoms,accompanied symptoms,tongue picture and pulse condition of the model group swine were all significantly changed compare with those in the control group respectively. Tongue picture of model group showed lower R value and red saturation level,the pulse wave revealed h3 / h1 difference after 2 weeks. Conclusion： The method of objective standardization scoring for TCM syndrome shows a better stability in the study of mini swine CHD model of phlegm-stasis syndrome type. The normalized tongue picture collection method contributes to evaluate the changes of disease,while the pulse wave pattern method in evaluating the pulse condition of compound factor animal model still needs to be investigated.