基于网络药理学探讨大黄牡丹汤治疗溃疡性结肠炎的作用机制

目的：分析大黄牡丹汤的有效化学成分及作用靶点，研究其治疗溃疡性结肠炎（Ulcerative Colitis,UC）的作用机制。方法：运用中药系统药理学数据库与分析平台(TCMSP)检索大黄牡丹汤的活性成分及靶点蛋白；应用基因组注释数据库平台（Genecards）预测疾病的作用靶点，再将药物-靶点-疾病关系制作成网络图形式。通过String数据库平台构建蛋白质-蛋白质相互作用（PPI）网络，寻找PPI核心基因，再进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析，找出所涉及的信号通路，构建靶点-通路网络图。结果：通过筛选，搜索出大黄牡丹汤治疗UC的关键化学成分18个，共同靶点28个，得到15个PPI核心基因，分别为MYC、JUN、CASP3、ESR1、PTGS2、HSP90AA1、CASP8、CASP9、IL1B、CCNB1、CDKN1A、CHEK1、PPARG、BAX、KDR。GO富集分析发现紫外线应答、膜筏、半胱氨酸型内肽酶活性参与凋亡过程等；KEGG富集通路分析发现P53信号通路、小细胞肺癌信号通路、乙型肝炎信号通路、大肠癌信号通路等。结论：大黄牡丹汤可通过调节PTGS2、PTGS1、HSP90AA1、BAX、CASP3、ESR1、ESR2、FASN、JUN、NOS2等靶点，调控P53信号通路等来抑制炎症反应、调节免疫功能对UC起到治疗作用。

Mechanism of Dahuang Mudan Decoction in the Treatment of Ulcerative Colitis Based on Internet Pharmacology

To analyze the effective chemical components and action targets of Dahuang Mudan Decoction,and to study the mechanism of its treatment for ulcerative colitis(UC).Methods:Chinese Medicine System Pharmacology Technology Platform(TCMSP) database was used to search for the active ingredients and target proteins of Dahuang Mudan Decoction; the genomic annotation database platform(Genecards)was used to predict the target of the disease,and then make the drug-target-disease relationship into a network Graphic form; the PPI network was constructed using the String database platform,and we searched for PPI core genes,and then performed GO enrichment analysis and KEGG enrichment analysis to find out the signal pathways involved and constructed a target-path network graph.Results:After screening,a total of 18 key chemical components of Dahuang Mudan Decoction for UC were obtained,and 28 common targets were obtained,and 15 PPI core genes were obtained,namely MYC,JUN,CASP3,ESR1,PTGS2,HSP90AA1,CASP8,CASP9 IL1B,CCNB1,CDKN1A,CHEK1,PPARG,BAX,KDR; GO enrichment analysis results included ultraviolet stress,membrane raft,cysteine-type endopeptidase activity involved in the apoptosis process,etc; KEGG enrichment pathways included P53 signaling pathway,small cell lung cancer signaling pathway,hepatitis B signaling pathway,and colorectal cancer signaling pathway.Conclusion:Dahuang Mudan Decoction can inhibit inflammation and regulate immune function by regulating the targets of PTGS2,PTGS1,HSP90AA1,BAX,CASP3,ESR1,ESR2,FASN,JUN,NOS2,etc.to suppress inflammation and regulate immune function.