黄连解毒汤对胰岛素抵抗大鼠骨骼肌IRS-1表达及酪氨酸磷酸化水平的影响

目的:观察黄连解毒汤对大鼠骨骼肌胰岛素受体底物1(IRS-1)表达及其磷酸化水平的影响,探讨其改善胰岛素抵抗的分子机制.方法:采用小剂量链脲佐菌素尾静脉注射加高糖高脂饲料喂养方法建立Wistar大鼠胰岛素抵抗模型,以黄连解毒汤干预治疗10周,检测血糖和血清胰岛素,用免疫沉淀和Western印迹方法检测黄连解毒汤治疗后胰岛素抵抗大鼠骨骼肌组织内IRS-1蛋白表达水平和酪氨酸磷酸化水平的改变.结果:黄连解毒汤治疗后,胰岛素抵抗大鼠肌肉组织内IRS-1表达较模型组增加,IRS-1酪氨酸磷酸化水平较模型组显著增加.结论:黄连解毒汤提高胰岛素抵抗大鼠骨骼肌组织IRS-1蛋白表达和酪氨酸磷酸化水平,这可能是其降血糖并改善组织胰岛素敏感性的机制之一.

Effects of Huanglian Jiedu Decoction on IRS-1 Expression and Tyrosine Phosphorylation Level in Skeletal Muscles of the Rats with Insulin Resistance

Objective:To observe effects of Huanglian Jiedu Decoction on expression of insulin receptor substrate 1(IRS-1)and the level of tyrosine phosphorylation in skeletal muscles to explore the molecular mechanism in improving insulin resistance.Methods:Wistar system insulin resistance model rats were developed with injection of small dose of streptozotocin into the caudal vein plus feeding by forage with high sugar and high lipids,and they were treated with Huanglian Jiedu Decoction for 10 weeks.Then blood sugar and serum insulin level were determined,and the changes of IRS-1 protein expression and tyrosine phosphorylation level in the skeletal muscles of the rat with insulin resistence after treatment of Huanglian Jiedu Decoction were detected with immuno- precipitation and Western Blot methods.Results:After treatment with Huanlian Jiedu Decoction,IRS-1 expression and IRS-1 tyrosine phosphorylation level in the muscular tissue increased as compared with those of the model group.Conclusion Huanglian Jiedu Decoction improves IRS-1 protein expression and tyrosine phosphorylation level in the skeletal muscle tissue of the rats with insulin resistance,which is possibly one of the mechanisms of decreasing blood sugar and improving sensitivity of insulin in tissues.