颈复康颗粒对颈椎动静力失衡大鼠颈椎间盘形态学改变的影响

目的 观察颈复康颗粒对颈椎动静力失衡大鼠颈椎间盘形态学改变的影响.方法 将SD雄性大鼠随机分成对照组、模型组、颈复康颗粒组、莫比可组各12只,建立动静力失衡性大鼠颈椎间盘退变模型,给药4周后每组取4个标本2张连续切片,观察组织病理学改变,测量软骨终板非钙化层与钙化层厚度比值、软骨终板内血管芽数量,检测各组退变椎间盘血管内皮细胞生长因子(VEGF)、胰岛素样生长因子1(IGF-1)、转化生长因子β(TGF-β)表达.结果与对照组比较,模型组大鼠颈椎间盘出现退行性改变,软骨与椎体交界面血管芽数量减少,软骨终板非钙化层与钙化层比值下降,大鼠颈椎间盘中VEGF表达增加、IGF-1和TGF-β表达下降(P＜0.01);与模型组比较,颈复康颗粒组血管芽数量增加(P＜0.01),颈复康颗粒组及莫比可组椎间盘组织形态学改善(P＜0.01或P%0.05),软骨终板非钙化与钙化层厚度比值增加(P＜0.01),大鼠颈椎间盘中VEGF表达下降、IGF-1和TGF-β表达增加(P＜0.01).结论 颈复康颗粒通过调节细胞因子VEGF、IGF-1、TGF-β的表达,改善大鼠颈椎间盘退变模型形态学的表现,从而减少椎间盘细胞外基质降解,延缓椎间盘退变.

Effects of Jingfukang Granule on the Morphological Changes of Cervical Intervertebral Disc in Rats with Cervical Intervertebral Disc Degeneration Induced by Unbalanced Dynamic and Static Forces

Objective To investigate the effect of Jingfukang Granule(Granule for cervical spondylosis)on the morphological changes of cervical intervertebral disc in rats with cervical intervertebral disc degeneration induced by unbalanced dynamic and static forces.Methods Male SD rats were randomly divided into control group,model group,Jingfukang Granule group,and Mobic group,12 rats in each group.The imbalance model of dynamic and static force of cervical intervertebral disc degeneration waft established.The drugs were given after modeling,and the rats were sacrificed 4 weeks later.Four samples of cervical intervertebral disc were collected from each group and two sections in succession were used to observe the histopathological changes.The ratio of un-calcifled layer to calcified layer in cartilage endplate,and the number and area of vascular bud were measured under light microscope.Expression of vascular endothelial growth factor(VEGF),insulin-like growth factor-1(IGF-1),and transforming growth factor-β(TGF-β)were tested.Results Rats in the model group had degeneration in the cervical intervertebral disc,decrease of the number of vascular bud,and decrease of the ratio of un-calcified layer to calcified layer in cartilage endplate,increased VEGF expression,and decreased IGF-1 and TGF-β expressions(P＜0.01).Compared with the model group,the rats in the Jingfukang Granule group had increased number of vascular bud(P＜0.01);the rats in the Jingfukang Granule group and Mobic group had improved morphological structure(P＜0.01 or P＜0.05),increased ratio of un-calcified layer to calcified layer in cartilage endplate(P＜0.01),down-regulated expression of VEGF,and up-regulated expression of IGF-1 and TGF-β(P＜0.01).Conclusion By regulating the expression of VEGF,IGF-1,and TGF-β,Jingfukang Granule could improve the morphological changes.With the decreased degradation of extracellular matrix,the degeneration of intervertebral disc could be delayed.