防哮饮早期干预对哮喘小鼠嗜酸性粒细胞和气道炎症的影响

目的 观察防哮饮早期干预对哮喘气道炎症的防治作用机制. 方法 将50只BALB/c健康小鼠随机分为正常对照组、哮喘模型组、防哮饮小剂量组、防哮饮大剂量组、布地奈德(BUD)组,每组10只,用卵蛋白(OVA)致敏激发造模.防哮饮小剂量组、大剂量组从小鼠第1次致敏当天起分别灌胃给予防哮饮500g/L、2 500g/L,每天1次,每次0.4ml,共26天.正常对照组和哮喘模型组则以等量生理盐水灌胃.BUD组于第23天予BUD溶液(0.5g/L)0.01ml/d,滴鼻3天.最后1次激发后48h处死小鼠,检测小鼠血清白细胞介素4(IL-4)、白细胞介素5(IL-5)、γ-干扰素(IFN-γ)、白细胞介素12(IL-12)的含量,光镜下观察肺部EOS浸润和骨髓EOS百分计数(EOS％).结果 哮喘模型组小鼠肺组织HE染色显示小鼠肺部出现大量的炎症细胞浸润,以EOS和中性粒细胞为主.哮喘模型组骨髓EOS％、血清IL-4/IFN-γ的比值、IL-5/IL-12的比值较正常对照组明显升高(P＜0.01)；防哮饮大剂量组和BUD组均能显著降低骨髓EOS％、明显抑制气道及血管周围炎症浸润、降低哮喘小鼠血清IL-4/IFN-γ和IL-5/IL-12的比值(P＜0.05或P＜0.01),两组比较差异无统计学意义(P＞0.05). 结论 防哮饮能有效下调哮喘小鼠血清IL-4/IFN-γ和IL-5/IL-12的比值,降低骨髓EOS％,抑制以EOS浸润为主的气道炎症.

Effect of Early Intervention with Fangxiaoyin Granules on Eosinophils and Airway Inflammation in Asthmatic Mice

Objective To investigate the effect and mechanism of early intervention with Fangxiaoyin Granules (Granules for preventing asthma) on airway inflammation in asthmatic mice.Methods Fifty healthy BALB/c mice were randomized into 5 groups,i.e.normal control group,asthma model group,Fangxiaoyin Granules small-dose group,Fangxiaoyin Granules large-dose group,and budesonide (BUD) group,with 10in each.The asthmatic models were established with ovalbumin (OVA) sensitization for inspiration.The Fangxiaoyin Granules small-dose group and large-dose group were given small-dose (500g/L crude medicine) and large-dose (2500g/L crude medicine) of Fangxiaoyin Granules respectively through gastric feeding from the first day of sensitization,once a day,0.4ml each time,26days in all.The normal control group and asthma model group were given normal saline in the equal amount through gastric feeding.The BUD group was given BUD solution (0.5g/L) for nasal dripping since the 23rd day under the ethyl ether inhalation anesthesia,0.01ml/day,for 3days.Forty-eight hours after the last inspiration,the mice were put to execution.The contents of serum interleukin-4 (IL-4),interleukin-5 (IL-5),interfer-on-γ (IFN-γ),and interleukin-12 (IL-12) were measured,and the eosinophils (EOS) infiltration in the lung and marrow EOS% were observed with photoelectric microscope.Results In the asthma model group,the HE dyeing of the lung tissues showed that there were massive inflammatory infiltration,mainly EOS and neutrophils;the marrow EOS%,serum IL-4/IFN-γratio,and IL-5/IL-12ratio were significantly increased in comparing with those of the normal control group (P<0.01).In the Fangxiaoyin Granules large-dose group and BUD group,the marrow EOS% was significantly decreased,the airway and perivascular inflammatory infiltration was significantly inhibited,and serum IL-4/IFN-γratio and IL-5/IL-12ratio were significantly decreased,the difference between the two groups was not statistically significant (P>0.05).Conclusion Fangxiaoyin Granules could effectively down-regulate serum IL-4/IFN-γratio and IL-5/IL-12ratio,decreasing the marrow EOS%,and inhibiting the airway inflammation mainly with EOS infiltration.