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| 补肾调冲方对卵巢早衰大鼠卵泡发育的影响 | |
| 引用本文： | 杨涓,高慧,夏天,韩冰,董江川.补肾调冲方对卵巢早衰大鼠卵泡发育的影响[J].中医杂志,2011,52(7):592-595. |
| 作者姓名： | 杨涓 高慧 夏天 韩冰 董江川 |
| 作者单位： | 1. 重庆医科大学附属第一医院,重庆市渝中区友谊路1号,400016 2. 河北承德医学院附属医院 3. 天津中医药大学第一附属医院 4. 天津中医药大学第二附属医院 5. 重庆医科大学中医药学院 |
| 基金项目： | 河北省中医药管理局科研项目 |
| 摘 要： | 目的从卵泡发育调控的旁分泌途径研究补肾调冲方的作用机制。方法将60只SD大鼠随机分为正常组,模型组,补肾调冲方高、中、低剂量组及西药组,每组10只,采用雷公藤多苷灌胃(50mg/kg,14天)建立卵巢早衰模型,分别使用高(1ml/100g)、中(0.5ml/100g)、低(0.25ml/100g)剂量补肾调冲方及倍美力(0.075ml/100g)进行干预,时间为35天,观察各组大鼠各级卵泡及黄体计数和卵巢超微结构改变,检测卵巢卵泡刺激素受体(FSHR)、表皮生长因子(EGF)及缝隙连接蛋白43(Cx43)的mRNA表达。结果模型组各级卵泡数、黄体数明显减少,卵巢超微结构受损,EGF、Cx43mRNA表达显著降低(P<0.05或P<0.01),补肾调冲方中、低剂量组及西药组大、中卵泡数及黄体数明显增加,卵巢超微结构明显改善,EGF及Cx43的mRNA表达显著增高(P<0.05或P<0.01)。结论补肾调冲方可改善雷公藤多苷所致的卵泡发育障碍,其作用机制可能是通过促进卵巢EGF、Cx43mRNA表达这一旁分泌调控途径实现。 |
| 关 键 词： | 补肾调冲方 卵巢早衰 卵泡刺激素受体 表皮生长因子 缝隙连接蛋白43 |
Effect of Bushen Tiaochong Formula on Follicular Development in Ovarian Senilism Rats |
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| YANG Juan,GAO Hui,XIA Tian,HAN Bing,DONE Jiangchuan.Effect of Bushen Tiaochong Formula on Follicular Development in Ovarian Senilism Rats[J].Journal of Traditional Chinese Medicine,2011,52(7):592-595. | |
| Authors: | YANG Juan GAO Hui XIA Tian HAN Bing DONE Jiangchuan |
| Institution: | YANG Juan1,GAO Hui2,XIA Tian3,HAN Bing4,DONG Jiangchuan5(1.The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,2.The Affiliated Hospital of Chengde Medical University,3.The First Affiliated Hospital of Tianjin University of Chinese Medicine,4.The Second Affiliated Hospital of Tianjin University of Chinese Medicine,5.College of Chinese Medicine,Chongqing Medical University) |
| Abstract: | Objective To investigate the regulatory mechanism of Bushen Tiaochong Formula(Formula for reinforcing the kidney and regulating the Chong meridian)on paracrine pathway of follicular development.Methods A total of 60 SD rats were randomized into normal group,model group,Bushen Tiaochong Formula large-dose group,medium-dose group,small-dose group,and western medicine group,with ten in each.Except for the normal group,the other groups were treated with glycoside of tripterygium wilfordii(GTW)by gavage(50mg/kg)... |
| Keywords: | Bushen Tiaochong Formula(Formula for reinforcing the kidney and regulating the Chong meridian) Ovarian senilism Follicle stimulating hormone receptor(FSHR) Epidermal growth factor(EGF) Connexin 43(CX43) |
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