疏肝润肠方防治慢传输型便秘可能机制及效应靶点的探讨

目的]探讨疏肝润肠方干预慢传输型便秘可能机制及效应靶点.方法]60只SPF级Balb/c雄性小鼠,按体质量随机分为:正常组、模型组、治疗组(含低、中、高剂量亚组).除正常组外,模型组、治疗组均以复方地芬诺酯灌胃制模.观察所有小鼠排便时间、排便次数及粪重,应用免疫组化SABC法检测Cajal细胞标志物C-kit表达,实时荧光定量PCR技术检测血浆中P物质(SP)和血管活性肠肽(VIP) mRNA表达变化.结果]模型组大便次数减少、排便时间延长、粪便少而且硬；治疗组较模型组大便次数增加、排便时间缩短、大便性状偏软(P＜0.05).与正常组比较,模型组小鼠结肠黏膜Cajal细胞阳性分布面积及数目减少(P＜0.05).模型组小鼠结肠SP、VIP mRNA表达呈下降趋势；治疗组小鼠结肠SP、VIP mRNA表达呈上升趋势(P＜0.05).结论]疏肝润肠方可能通过调控SP及VIP的分泌,从而缩短慢传输型便秘小鼠排便时间、增加排便次数及便重、促进小鼠粪便软化,增加肠推进,促进小肠的传输功能而发挥其治疗慢传输型便秘的药效.

Changes of Cajal cell and gastrointestinal hormone in colon mucosain mice of slow transit constipation treated with Shugan Runchang decoction

Objective]To investigate the effect and the possible mechanism of Shugan Runchang decoction(SRD)in treating slow transit constipation(STC).Methods]Sixty SPF male Balb/c mice were randomly divided into five groups:normal group,model group,SRD intervention group of low,middle and high dosage.After a week of adaption,except the normal group,the other groups were administrated with compound Diphenoxylate tablets to tablish STC models.From the second day,the mice were given normal saline and different dosage SRD respectively for five days.All mice were sacrificed by decapitation at the end of the seventh day.The manifestations of STC including the duration of defecation,defecation frequency and average stool weight,character of stool were observed.The expression of Cajal cells were assayed by immunohistochemistry method.The changes of SP and VIP were detected by Real-time PCR.Results]The times of defecation and the average stool weight decreased and the duration of defecation increased in the model group.SRD intervention improved the above manifestations significantly(P<0.05).The expression of Cajal cells reduced in the model group.The expression of SP and VIP mRNA in model group deceased,which were increased significantly by the intervention of SRD(P <0.05)Conclusion]SRD is effective in treating STC,which might be related to shortening defecation duration,increase of defecation frequency and the average stool weight through the regulation of SP and VIP secretion.