| 抗纤软肝颗粒对血小板源生长因子诱导的肝星状细胞增殖的影响 |
| |
| 引用本文： | 杨玲,张赤志,朱清静,明安萍.抗纤软肝颗粒对血小板源生长因子诱导的肝星状细胞增殖的影响[J].中国中西医结合消化杂志,2004,12(2):80-82. |
| |
| 作者姓名： | 杨玲 张赤志 朱清静 明安萍 |
| |
| 作者单位： | 1. 华中科技大学同济医学院附属协和医院中医科,武汉,430022
2. 湖北中医学院附属医院 |
| |
| 基金项目： | 湖北省自然科学基金，湖北省教育厅科研项目 |
| |
| 摘 要： | 目的:研究抗纤软肝颗粒(KXR)对血小板源生长因子(PDGF)诱导的肝星状细胞(HSC)增殖的影响.方法:采用无血清培养,不同浓度的KXR温育HSC 24 h后,PDGF-BB(10 ng/ml)刺激24 h,再加入上述浓度的KXR 3 h后,又加入PDGF-BB(10 ng/ml)作用5 min,然后收集细胞.采用细胞计数法及流式细胞仪测定细胞增殖及细胞周期.结果:无血清培养显示该方对于PDGF诱导的细胞增殖具有抑制作用,并呈剂量依赖性(P<0.01).流式细胞术分析提示KXR能抑制PDGF诱导的HSC DNA合成期及合成后期和分裂期DNA百分含量,阻断细胞由静止期/DNA合成前期向DNA合成期转化,呈剂量依赖性(5 mg/ml组作用最显著,P<0.01).结论:KXR能抑制PDGF诱导的HSC增殖.
|
| 关 键 词： | 抗纤软肝颗粒 肝纤维化 活血化瘀 肝星状细胞 血小板源生长因子 |
Effect of Kangxian Ruangan Granule on Proliferation of Hepatic Stellate Cells Induced by Platelet-derived Growth Factor |
| |
| NG Ling,ZHANG Chizhi,ZHU Qingjing,et al
TCM.Effect of Kangxian Ruangan Granule on Proliferation of Hepatic Stellate Cells Induced by Platelet-derived Growth Factor[J].Chinese Journal of Integrated Traditional and Western Medicine on Digestion,2004,12(2):80-82. |
| |
| Authors: | NG Ling ZHANG Chizhi ZHU Qingjing TCM |
| |
| Institution: | NG Ling,ZHANG Chizhi,ZHU Qingjing,et al
TCM Department,Union Hospital Affiliated to Tongji Medical College,Huazhong University of
Science and Technology,Wuhan 430022 |
| |
| Abstract: | jective:To investigate the effect of Kangxian Ruangan granule (KXR) on hepatic stellate cells(HSC) proliferation induced by platelet-derived growth factor ( PDGF) in vitro. Methods: Serum-free culture method was used. HSC were treated with KXR for 24 hours, and stimulated with PDGF-BB for 24 hours, and then cultured with the same concentration of KXR for 3 hours, stimulated with PDGF-BB for 5 minutes. The cells were collected and the proliferation of HSC was examined with cell counting method. The cell cycle was analyzed with flow cytometry. Results: Serum-free culture indicated KXR could attenuate HSC growth with dose-dependant tendency ( P < 0. 01). Flow cytometry analysis indicated KXR could inhibit DNA synthesis of S and G2/M phases induced by PDGF, block HSC cell cycle beyond G1 phase, which existed dose-dependent relation ( P <0. 01). Conclusion: KXR could inhibit the proliferation of HSC induced by PDGF. |
| |
| Keywords: | nxian Ruangan Granule liver fibrosis proliferation hepatic stellate cell plateletderived growth factor |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
