pS2/TFF1蛋白在胃癌及其癌前病变组织中表达的研究

背景:pS2/TFF1蛋白属于三叶因子家族,是一类具有胃肠道黏膜保护和修复作用的生长因子类小分子多肽物质,研究pS2/TFF1蛋白可能为胃癌的防治开辟新的思路。目的:观察pS2/TFF1蛋白在胃癌及其癌前病变组织中的表达,探讨其与胃癌发生的关系。方法:采用免疫组化法检测30例慢性非萎缩性胃炎、35例慢性萎缩性胃炎、50例慢性萎缩性胃炎伴肠化生、37例异型增生、46例胃癌和30名健康志愿者胃黏膜组织中pS2/TFF1蛋白的表达。结果:正常胃黏膜组织-慢性非萎缩性胃炎-慢性萎缩性胃炎-慢性萎缩性胃炎伴肠化生-异型增生-胃癌组织中,pS2/TFF1蛋白阳性表达呈逐渐下降的趋势(分别为100%、93.3%、82.9%、78.0%、62.2%、56.5%),差异有统计学意义(P0.05)。与正常对照组、慢性非萎缩性胃炎组和慢性萎缩性胃炎组相比,慢性萎缩性胃炎伴肠化生组、异型增生组和胃癌组pS2/TFF1蛋白阳性表达均显著降低(P0.05),而后三组之间的差异无统计学意义(P0.05)。结论:pS2/TFF1蛋白表达降低是胃癌发生过程中的早期事件,有望成为诊断胃癌的标记物。

Study on Expression of pS2/TFF1 Protein in Gastric Cancer and Precancerous Lesion

Background： pS2/TFF1 protein, a member of trefoil factor family, is a growth factor like micromolecule polypeptide playing protection and repair roles in gastrointestinal tract mucosa, and study on pS2/TFF1 protein may give some highlights to the prevention and treatment of gastric cancer. Aims： To investigate the expression of pS2/TFF1 protein in gastric cancer and precancerous lesion, and to study the relationship between pS2/TFF1 protein and gastric cancer. Methods： Expression of pS2/TFF1 protein in gastric mucosa was determined by immunohistochemistry in 30 chronic non-atrophic gastritis, 35 chronic atrophic gastritis, 50 chronic metaplastic atrophic gastritis, 37 dysplasia, 46 gastric cancer and 30 healthy volunteers. Results： The positivity rates of pS2/TFF1 protein expression in normal controls, chronic non-atrophic gastritis, chronic atrophic gastritis, chronic metaplastic atrophic gastritis, dysplasia and gastric cancer were 100%, 93.3%, 82.9%, 78.0%, 62.2%, 56.5%, respectively, the tendency of decrease was statistically significant （/）〈0.05）. Compared with normal control group, chronic non-atrophic gastritis group and chronic atrophic gastritis group, positivity rates of pS2/TFF1 protein expression in chronic metaplastic atrophic gastritis group, dysplasia group and gastric cancer group was significantly decreased （P〈0.05）, however, no significant difference was found among the latter three groups （P〉0.05）. Conclusions： Decreased expression of pS2/TFF1 protein is an early molecular event in the development of gastric cancer. pS2/TFF1 protein has the potential to be a marker for the diagnosis of gastric cancer.