几种培养幽门螺杆菌方法的比较

背景：全球50％以上的人口感染幽门螺杆菌（H.pylori），对其培养方法的研究非常重要。目的：研究不同培养基对H.pylori生长的影响。方法：制备固体和液体培养基，并加入脱纤维绵羊血和3种动物血清（羊、马、牛）行且pytori培养。以细菌形态、涂片镜检、快速尿素酶试验、PCR法和菌体浓度鉴定H.pylori。结果：添加脱纤维绵羊血和动物血清的固体和液体培养基均可成功培养出且pylori，其中脱纤维绵羊血的固体培养基扩菌浓度最高．添加马血清的固体和液体培养基的H．pylori扩菌效果均较理想。结论：固体和液体培养基均可取得较为满意的H.pylori扩菌效果．其中添加马血清的培养基更易于分装和保存．值得推广．

Comparison of Several Culture Methods for Helicobacter pylori

Background: Helicobacter pylori(H.pylori) is infected in more than half of population in the world,and it is very important to study the culture methods for H.pylori.Aims: To study the effect of different culture medium on growth of H.pylori.Methods: Solid and liquid culture medium were prepared with defibrinated sheep blood and 3 kinds of animal serum(sheep,horse,cattle) for culture of H.pylori.Morphology of bacteria colony,microscopic examination,rapid urease test,PCR assay and concentration of bacteria were used to identify H.pylori.Results: Solid and liquid medium containing defibrinated sheep blood and animal serum could successfully culture H.pylori.H.pylori concentration was the highest in solid media containing defibrinated sheep blood,and culture medium containing horse serum were more satisfactory for the culture of H.pylori.Conclusions: Solid and liquid culture medium can obtain satisfactory culture of H.pylori.Culture medium containing horse serum is convenient for allocation and storage and is worthy to be widespread use.