| 肺癌细胞裂解物负载对树突状细胞分泌的exosome诱导抗肿瘤作用的影响 |
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| 引用本文： | 张在云,李希德,刘叶,王志仑,潘祥林.肺癌细胞裂解物负载对树突状细胞分泌的exosome诱导抗肿瘤作用的影响[J].山东医药,2011,51(31):4-6. |
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| 作者姓名： | 张在云 李希德 刘叶 王志仑 潘祥林 |
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| 作者单位： | 1. 山东大学第二医院,济南,250033
2. 高密县中医院 |
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| 基金项目： | 山东省自然科学基金资助项目(Y2007C111) |
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| 摘 要： | 目的为制备高效的胞外体（exosome）肿瘤疫苗提供理论依据。方法用细胞因子诱导培养树突状细胞（DC），将肺癌细胞裂解物负载DC，提取exosome用exosome活化T细胞（负载组），以未负载DC的exosome（未负载组）及肺癌细胞裂解物负载DC（DC组）活化的T细胞为对照，MTr法检测三组肺癌细胞的杀伤率。结果exosome中有HSP70、HLA及CEA表达。活化T细胞／肺癌细胞为25：1、10：1、5：1时负载组杀伤率均明显高于未负载组及DC组（P均〈0．05）。结论肺癌细胞裂解物负载能增强DC分泌的exosome诱导的抗肿瘤作用；本研究为制备高效的exosome肿瘤疫苗提供了理论依据。
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| 关 键 词： | 肺肿瘤 树突细胞 胞外体 肿瘤疫苗 |
Anti-tumor effect induced by exosomes derived from dendritic cells loaded with lung cancer cell lysates |
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| ZHANG Zai-yun,LI Xi-de,LIU Ye,WANG Zhi-lun,PAN Xiang-lin.Anti-tumor effect induced by exosomes derived from dendritic cells loaded with lung cancer cell lysates[J].Shandong Medical Journal,2011,51(31):4-6. |
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| Authors: | ZHANG Zai-yun LI Xi-de LIU Ye WANG Zhi-lun PAN Xiang-lin |
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| Institution: | ZHANG Zai-yun1,LI Xi-de,LIU Ye,WANG Zhi-lun,PAN Xiang-lin(1 The Second Hospital of Shandong University,Jinan 250033,P.R.China) |
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| Abstract: | Objective To obtain theoretical bases for making high efficacy exosome cancer vaccine.Methods Dendritic cells(DC) were induced with cytokines and then loaded with whole lung cancer cell lysates.Exosomes were isolated from supernatant of DC,and T cells activated by the exosomes(group loaded),T cells activated by exosomes from non-loaded DC(group non-loaded) or activated by lysate-loaded DC(group DC) were taken as control.The activity of T cells for killing lung cancer cells were detected by MTT method.Result... |
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| Keywords: | lung neoplasms dendritic cells exosome cancer vaccine |
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