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| ERK-1/2对N/OFQ调节大鼠皮层神经元延迟整流钾电流激活动力学的影响 | |
| 引用本文： | 李晓红,王艳丽,张宇,刘程伟,曲丽辉.ERK-1/2对N/OFQ调节大鼠皮层神经元延迟整流钾电流激活动力学的影响[J].山东医药,2012,52(12):51-53. |
| 作者姓名： | 李晓红 王艳丽 张宇 刘程伟 曲丽辉 |
| 作者单位： | 1. 哈尔滨医科大学附属第一医院,哈尔滨,150001 2. 哈尔滨医科大学 3. 黑龙江省医院 4. 佳木斯大学附属第一医院 |
| 基金项目： | 国家自然科学基金-青年科学基金项目,国家自然科学基金资助项目 |
| 摘 要： | 目的研究ERK-1/2对N/OFQ调节大鼠皮层神经元延迟整流钾电流(IK)激活动力学的影响,初步探讨N/OFQ调节大鼠皮层神经元IK的ERK-1/2途径。方法研究采用全细胞膜片钳技术,观察ERK-1/2特异性抑制剂U0126对N/OFQ调节大鼠皮层神经元IK激活动力学的影响。结果在ERK-1/2抑制剂U0126存在情况下,IK激活曲线的V1/2由给药前的(-42±3.7)mV向超极化方向移动为(-39.9±5.9)mV(P<0.01)。IK激活曲线的κ由给药前的(21.6±8.1)mV变为给药后的(22.9±3.8)mV(P<0.05)。结论 U0126可以显著抑制N/OFQ对IK激活曲线的影响。 |
| 关 键 词： | ERK-1/2 延迟整流钾电流 全细胞膜片钳技术 孤啡肽 大鼠 |
Effects of ERK-1/2 on the modulation of delayed rectifier potassium current activation kinesics by N/OFQ in acutely dissociated rat cerebral parietal cortical neurons |
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| LI Xiao-hong , WANG Yan-li , ZHANG Yu , LIU Cheng-wei , QU Li-hui.Effects of ERK-1/2 on the modulation of delayed rectifier potassium current activation kinesics by N/OFQ in acutely dissociated rat cerebral parietal cortical neurons[J].Shandong Medical Journal,2012,52(12):51-53. | |
| Authors: | LI Xiao-hong WANG Yan-li ZHANG Yu LIU Cheng-wei QU Li-hui |
| Institution: | 1 The First Affiliated Hospital of Harbin Medical University,Harbin 150001,P.R.China) |
| Abstract: | Objective To investigate the effects of ERK-1/2 on modulation of delayed rectifier potassium current(IK) activation kinesics by N/OFQ in acutely dissociated rat cerebral parietal cortical neurons.Method Whole-cell voltage clamp technique was used.Result In presence of U0126,inhibitor of ERK-1/2,0.1 μmol/L N/OFQ shifted the activation curve of IK to hyperpolarize potential from(-42 ± 3.7) mV to(-39.9 ± 5.9) mV(P<0.01) and changed the slope factor(κ) of the activation curve from(21.6 ± 8.1) mV to(22.9 ± 3.8) mV(P<0.05).Conclusion These data sustained our contention that U0126 inhibited the positive shift of IK activation induced by N/OFQ. |
| Keywords: | ERK-1/2 delayed rectifier potassium current whole-cell voltage clamp technique orphanin FQ/nociceptin rat |
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