动脉瘤夹不同夹闭时间对兔颈总动脉管壁的影响

目的探讨一次性使用永久动脉瘤夹不同夹闭时间对兔颈总动脉管壁的影响。方法选用健康雄性日本大耳白兔60只,按数字随机法分为两组,一次性使用永久动脉瘤夹分别夹闭颈总动脉30 min和60 min,每组各30只,分别取动脉瘤夹夹持部位颈总动脉标本30段,采用Mann-Whitney U检验,进行血管壁组织病理学损伤程度的比较。结果一次性使用动脉瘤夹夹闭30 min和60 min所致的血管壁损伤均可见中层弹力板形变和内皮细胞脱落,夹闭时间60 min组血管壁出现局部坏死伴发炎性反应,甚至管壁破裂。夹闭30 min组和夹闭60 min组血管壁总体损伤程度(U=324.00,P=0.045)和重度损伤率0%(0/30)比20.0%(6/30),P=0.031]差异均有统计学意义。结论一次性使用永久动脉瘤夹夹闭时间60 min比30 min对血管壁的损伤更重,术中应注意缩短夹闭颈动脉时间。

Effects of different clipping time of aneurysm clips on common carotid artery wall in rabbits

Objective To investigate the effects of different clipping time of first time using permanent aneurysm clips on common carotid artery wall in rabbitsMethods Sixty healthy male Japanese white rabbits were selected.The first time permanent aneurysm clips were used to clip common carotid artery for 30 min or 60 min respectively according to the random number method (n=30 in each group).Thirty segments of common carotid artery specimens clipped by aneurysm clips were collected respectively.Mean-Whitney U test was used to conduct the comparison of histopathological damage grade of vascular wall.Results The aneurysm clips were use to clip 30 min and 60 min caused vascular wall injury could observe the middle elastic plastic plate deformation and endothelial denudation.The vascular walls in the clipping 60 min group had local necrosis with inflammatory response,and even rupture of vascular wall.There were significant differences in overall damage degree of vascular wall (U=324.00,P=0.045) and severe injury rate (0%0/30] vs.20.0%6/30],P=0.031) between the clipping 30 min group and the clipping 60 min group (all P<0.05).Conclusion The vascular wall injury of using disposable permanent aneurysm clips for clipping 60 min was more severe than 30 min.Attention should be paid to shortening the time of carotid artery occlusion in operation.