蛋白激酶Cη基因A1427C多态性与动脉粥样硬化性脑梗死的关系

目的 探讨江苏省汉族人群蛋白激酶Cη（PKCη）基因A1427C单核苷酸多态性与动脉粥样硬化性脑梗死的关系。方法 选择120例动脉粥样硬化性脑梗死患者,采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的方法检测PKCη基因A1427C多态性、采用颈动脉彩色多普勒超声检测颈总动脉内膜-中层厚度,并与对照组100例年龄、性别相匹配的非神经科住院患者和健康体检者比较。将所有研究对象的脑梗死危险因素做多元Logistic回归分析。结果脑梗死组中C等位基因频率（40.8%）及CC＋AC基因型频率（65.0%）明显高于对照组（29.5%和49.0%）,P〈0.05;携带C等位基因的个体与非携带C等位基因的个体比较,患脑梗死的OR值为1.649,（95%CI：1.108-2.456）;与AA基因型比较,CC＋AC基因型患脑梗死的OR值为1.933,（95%CI：1.123-3.326）。脑梗死组颈总动脉内膜-中层厚度与对照组比较差异有统计学意义（P〈0.05）。Logistic回归分析显示,吸烟、高脂血症和C等位基因患脑梗死的OR值分别为2.102、2.004及1.857。结论PKCη基因A1427C多态性可能与动脉粥样硬化性脑梗死的发生相关,C等位基因可能是脑梗死发生的独立危险因素。

Relationship between single nuclestide polymorphisin A1427C in protein kinase Cη gene and athero sclerotic cerebral infarction

Objective To explore the relationship between single nucleotide polymorphism （SNP） A1427C in protein kinase Cη （PKCη） gene and atheroselerotie cerebral infarction in Han nationality popu- lation in Jiangsu, China. Methods A total of 120 patients with atheroselerotie cerebral infarction were selected, and SNP A1427C in PKCη gene was detected by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method, and the common carotid intima-media thickness was measured by carotid uhrasonography/Doppler. They were compared with age and sex matched 100 non-neurological inpatients and healthy individuals in a control group. The risk factors of cerebral infarction in all participants were evaluated by multivariate logistic regression analysis. Results The frequencies of C allele （40. 8% ） and CC ＋ AC genotypes （65. 0% ） in the cerebral infarction group were significantly higher than those in the control group （29. 5% and 49.0% ,P 〈 0.05 ） ; The individuals with C allele compared with the individuals without C allele, the odds ratio （OR） of developing cerebral infarction was 1. 649 （95% CI 1. 108 -2. 456）. As compared with the AA genotypes, the OR of developing cerebral infarction in the CC ＋ AC genotypes was 1. 933 （95% CI 1. 123 -3.326）. There was statistical difference between the cerebral infarction group and the control group in common carotid intima-media thickness （ P 〈 0. 05 ）. Logistic regression analysis indicated that smoking, hyperlipidemia and C allele were the independent risk factors for cerebral infarction in Han population in Jiangsu. Conclusion SNP A1427C in PKCη may be associated with the occurrence of atherosclerotic cerebral infarction. The C allele may be a independent risk factor for cerebral infarction.