趋化因子配体16对人胰腺癌细胞株PANC1生物学行为的影响

目的探讨趋化因子配体16（CXCL16）对人胰腺癌细胞PANC1生物学行为的影响。方法取对数生长期的人胰腺癌细胞PANC1，分别加入50、1013、200ng／ml的重组人CXCL16（rhCXCL16）和抗CXCL16抗体处理4h，以不加rhCLCL16作为对照。采用MTT法检测细胞增殖，采用Mqrtigel基质检测细胞的黏附率，采用Transwell小室检测细胞侵袭和迁移能力。结果100ng／ml rhCXCL16处理PANC1细胞后，细胞增殖、对基质的黏附率、侵袭和迁移能力分别为0．264±0．021、（91．4±8．6）％、1．246±0．216、1．361±0．276，其中细胞对基质的黏附率、侵袭和迁移能力均显著高于对照组的（20．6±3．2）％、0．259±0．013、0．199±0．008（P〈0．01），对细胞的增殖不影响；2130ng/ml rhCXCL16处理后，细胞对基质的黏附率、侵袭和迁移能力进一步提高到（92．1±6．3）％、1．511±0．174、1．600±0．208（P〈0．05）。结论CXCL16可诱导人胰腺癌细胞PANC1增强对基质的黏附性、侵袭性和迁移性。

Effect of CXCL16 on the biological behavior of human pancreatic cancer cell line PANC1

Objective To investigate the effect of CXCL16 on the biological behavior of human pancreatic cancer cells PANC1. Methods Exponentially growing PANC1 cells was exposed to different concentration of rhCXCL16 （50,100,200 mg/ml） and CXCL16 antibody for 4 h, and PANC1 without rhCLCL16 treatment was used as the control. The proliferation was determined by MTT method, adhesion rate was determined by Mqrtigel matrix, invasion and migration of PANC1 ceils were assayed by Transwell chamber. Results After 100 ng/ml rhCXCL16 treatment, proliferation, adhesion rate, invasion and migration of PANC1 cells were 0. 264 ± 0. 021, 991.4 ± 8.6）% ,1. 246 ± 0. 216, 1. 361 ± 0. 276, respectively; and the adhesion rate, invasion and migration were significantly higher than （20.6 ± 3.2） %, 0. 259 ± 0. 013, 0. 199 ± 0. 008 in the control group （ P 〈 0.01 ）, and without significant effect on proliferation. After 200 ng/ml rhCXCL16 treatment, proliferation, adhesion rate, invasion and migration of PANC1 cells were further improved. Conclusions rhCXCL16 could enhance the ability of adhesion, invasion and migration of PANCI cells.