肾上腺髓质素在急性坏死性胰腺炎并肝损伤大鼠肝组织中的表达

目的观察急性坏死性胰腺炎（ANP）并肝损伤大鼠肝组织中肾上腺髓质素（ADM）mRNA的表达变化。方法64只SD大鼠按完全随机法分为ANP组和对照组，每组32只。采用逆行胰胆管注射5％牛磺胆酸钠的方法制备ANP模型，对照组仅剖腹翻动胰腺后关腹。术后第3、6、12、24h分批处死大鼠，取血检测淀粉酶、丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）浓度及ADM水平。取胰腺和肝脏组织行组织病理学检查。应用荧光定量PCR法检测肝组织ADMmRNA表达。结果造模后6h，ANP组大鼠血清淀粉酶、ALT、AST浓度分别为（7229．20±968．30）、（174．20±28．04）、（657．69±139．01）U／L，显著高于对照组的（2036．00±291．95）、（104．25±22．11）、（419．67±86．28）U／L，差异均有统计学意义（P〈0．05或〈0．01）。ANP组胰腺、肝组织病理损伤随时间延长逐渐加重，12h时的病理评分为（11．60±1．51）、（2．60±0．89）分，显著高于同时点对照组的（1．20±0．77）、0分，两组差异均有统计学意义（P值均〈0．01）。ANP组大鼠血清ADM水平于造模后3h上升，12h达（38．53±6．25）pg／ml，高于同时点对照组的（28．99±3．92）pg／ml；肝组织ADMmRNA表达量在3h升高，6h达到3．00±1．49，显著高于同时点对照组的1．04±0．20。两组差异均有统计学意义（P〈0．05或〈0．01）。结论ANP大鼠早期肝组织ADMmRNA表达增多，且血清ADM水平升高。

Expression of adrenomedullin in hepatic tissue of rats with acute necrotizing pancreatitis associated hepatic injury

Objective To observe the expression of adrenomedullin （ADM） mRNA in hepatic tissue of rats with acute necrotizing pancreatitis （ANP） complicated with hepatic injury. Methods Sixty-four SD rats were randomly divided into control group and ANP group with 32 rats in each group. In ANP group, ANP model was induced by retrograde injection of 5% sodium taurocholate into biliopancreatic duct of rats. Rats in control group only received sham operation and pancreas manipulation. All the rats were sacrificed at 3, 6, 12, 24 h after the operation. The serum levels of amylase, alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and ADM were detected. Pathological changes in pancreatic and hepatic tissue were examined. The expressions of ADM mRNA in hepatic tissue were evaluated by fluorescence quantitative PCR. Results The serum concentrations of amylase, ALT, AST were （7229 ±968 ）, （ 174.2 ±28.0）, （ 657.7 ± 139.0） U/L, which were significantly higher than those in control group （ 2036 ± 292 ）, （ 104.3 ±22.1 ）, （419.± 86.3）U/L], and the difference between the two groups was statistically significant （P 〈0.05 or P 〈0.01）. Pathological injury of pancreas and liver tissue in ANP gradually aggravated with time, and thepathological scores at 12 h were （ 11.60 ± 1.51 ）, （ 2.60± 0.89）, which were significantly higher than those in control group （ 1.20 ± 0.77, 0 ）, and the difference between the two groups was statistically significant （P 〈0.01）. The serum concentrations of ADM in ANP group increased at 3 h after ANP induction, and reached （38.53 ± 6.25 ）pg/ml at 12 h, which was significantly higher than that in control group （28.99 ± 3.92） pg/ml] ; the concentrations of ADM in liver tissue increased at 3 h after ANP induction, and reached （3.00 ±1.49） at 6 h, which was significantly higher than that in control group （ 1.04± 0.20）, and the difference between the two groups was statistically significant （ P 〈 0.05 or P 〈 0.01 ）. Conclusions The expression of ADM mRNA in rat ＇s hepatic tissue increases in the early stage of ANP, and the serum concentration of ADM also increases.