Macrophages in human visceral adipose tissue: increased accumulation in obesity and a source of resistin and visfatin |
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Authors: | C A Curat V Wegner C Sengenès A Miranville C Tonus R Busse A Bouloumié |
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Institution: | (1) Institute of Cardiovascular Physiology, Johann Wolfgang Goethe University, Theodor-Stern Kai 7, 60590 Frankfurt am Main, Germany;(2) Department of Surgery I, Klinikum Offenbach, Offenbach, Germany;(3) Obesity Research Unit, INSERM U 586, Louis Bugnard Institute, Centre Hospitalier Universitaire Rangueil, Université Paul Sabatier, Toulouse, France |
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Abstract: | Aims/hypothesis Increased visceral white adipose tissue (WAT) is linked to the risk of developing diabetes.
Methods/results We showed by fluorescence activated cell sorting analysis that human visceral WAT contains macrophages, the proportion of
which increased with obesity. Selective isolation of mature adipocytes and macrophages from human visceral WAT by CD14 immunoselection
revealed that macrophages expressed higher levels of chemokines (monocyte chemotactic protein 1, macrophage inflammatory protein
1α, IL-8) and the adipokines resistin and visfatin than did mature adipocytes, as assessed by real-time PCR analysis. Moreover,
resistin and visfatin proteins were found to be released predominantly by visceral WAT macrophages. Macrophage-derived secretory
products stimulated phosphorylation of protein kinase B in human hepatocytes.
Conclusions/interpretation Resistin and visfatin might be considered to be proinflammatory markers. The increased macrophage population in obese human
visceral WAT might be responsible for the enhanced production of chemokines as well as resistin and visfatin. |
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Keywords: | Adipocytes Adipokines Akt AMP-activated protein kinase Chemokines Cytokines Inflammation Interleukins Liver MAP kinases |
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