Androgen receptors in cultured rat adipose precursor cells during proliferation and differentiation: regional specificities and regulation by testosterone |
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Authors: | Dieudonne M N Pecquery R Leneveu M C Jaubert A M Giudicelli Y |
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Institution: | (1) Department of Biochemistry, INSERM CJF (94-02), Faculty of Medicine Paris-Ouest, Université Paris V, C.H.I., 78303 Poissy Cedex, France |
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Abstract: | Different studies suggest that sex hormones affect adipose tissue metabolism and deposition. To investigate the possibility
that androgens may play a role in adipose tissue development, we have studied androgen receptors (AR) in rat adipose precursor
cells from two different anatomical fat deposits, one deep intraabdominal (epididymal) and one subcutaneous (inguinal) during
the proliferation and differentiation processes. AR were quantified by 3H]R1881 specific binding in whole cells and the nuclear fraction and were localized by immunocytofluorimetry in both the cytosol
and the nucleus. During the proliferative phase, total AR level decreased from D3 to D6. At confluence (D5), AR were higher
in epididymal (64±4 fmol/mg protein) than in subcutaneous (33±3 fmoles/mg proteins) preadipocytes and were up-regulated by
testosterone but not by 5α-dihydrotestosterone or by 17β-estradiol. At differentiation (D10-11), nuclear AR decreased by 50%
in both precursor fat cell populations when compared to the confluent state (D5) and AR were no more up-regulated but rather
down-regulated by testosterone. Because AR are present in preadipocytes and are differently regulated by testosterone depending
on the stage of proliferation and differentiation, this study suggests that testosterone may play a role in the control of
the adipogenic process. |
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Keywords: | Androgen receptors cultured preadipocytes regulation adipogenesis regional specificities |
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