Establishment of cell clones with different metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97

AIM To establish clone cells with different metastaticpotential for the study of metastesis-related mechanisms.METHODS Cloning procedure was performed on parentalhepetocellular carcinoma (HCC) cell line MHCC97,andbiological characteristics of the target clones selected byin vivo screening were studied.RESULTS Two clones with high (MHCC97-H) and low(MHCC97-L) metastatic potential were isolated from theparent cell line.Compared with MHCC97-L,MHCC97-H hadsmaller cell size (average cell diameter 43μm vs 50 μm)and faster in vitro and in vivo growth rate (tumor celldoubling time was 34.2 h vs 60.0 h).The main ranges ofchromosomes were 55-58 in MHCC97-H and 57-62 inMHCC97-L.Boyden chamber in vitro invasion assaydemonstrated that the number of penetrating cells throughthe artificial basement membrane was (37.5±11.0) cells/field for MHCC97-H vs (17.7±6.3)/field for MHCC97-L.The proportions of cells In G0-G1 phase,S phase,andG2-M phase for MHCC97-H/MHCC97-L were 0.56/0.65,0.28/0.25 and 0.16/0.10,respectively,as measured byflow cytometry.The serum AFP levels in nude mice 5 wkafter orthotoplc implantation of tumor tissue were (246±66)μg.L~(-1) for MHCC97-H and (91±66)μg.L~(-1) for MHCC97-L.The pulmonary metastatic rate was 100% (10/10) vs40% (4/10).CONCLUSION Two clones of the same geneticbackground but with different biological behaviors wereestablished,which could be valuable models forInvestigation on HCC metastasis.

Establishment of cell clones with different metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97

AIM: To establish clone cells with different metastatic potential for the study of metastasis-related mechanisms. METHODS: Cloning procedure was performed on parental hepatocellular carcinoma (HCC) cell line MHCC97, and biological characteristics of the target clones selected by in vivo screening were studied. RESULTS: Two clones with high (MHCC97-H) and low (MHCC97-L) metastatic potential were isolated from the parent cell line. Compared with MHCC97-L, MHCC97-H had smaller cell size (average cell diameter 43 microm vs 50 microm) and faster in vitro and in vivo growth rate (tumor cell doubling time was 34.2h vs 60.0h). The main ranges of chromosomes were 55-58 in MHCC97-H and 57-62 in MHCC97-L. Boyden chamber in vitro invasion assay demonstrated that the number of penetrating cells through the artificial basement membrane was (37.5 +/- 11.0) cells/field for MHCC97-H vs (17.7 +/- 6.3)/field for MHCC97-L. The proportions of cells in G0-G1 phase, S phase, and G2-M phase for MHCC97-H/MHCC97-L were 0.56/0.65, 0.28/0.25 and 0.16/0.10, respectively, as measured by flow cytometry. The serum AFP levels in nude mice 5wk after orthotopic implantation of tumor tissue were (246 +/- 66) microg.L(-1) for MHCC97-H and (91 +/- 66) microg.L(-1) for MHCC97-L. The pulmonary metastatic rate was 100% (10/10) vs 40% (4/10). CONCLUSION: Two clones of the same genetic background but with different biological behaviors were established, which could be valuable models for investigation on HCC metastasis.