2型糖尿病患者动脉内中膜厚度与磷酸烯醇式丙酮酸羧激酶基因变异的相关性

目的探讨与2型糖尿病患者动脉内中膜厚度(TMT)进展与磷酸烯醇式丙酮酸羧激酶(PEPCK)基因rs2071023位点的变异的相关性。方法新诊断2型糖尿病患者121例超声测量颈动脉、股动脉和髂动脉的IMT,多聚酶链反应一限制性内切酶片段长度多态性技术(PCR-RFLP)检测基因型,比较不同基因型IMT进展的情况,随访5年后复查IMT。结果基线状态下rs2071023位点不同基因型(CC,CG,GG)各组年龄、IMT、BM、FPG、HbAlc、血脂比较差异无统计学意义。5年后随访患者中有16例失访或指标不全,共105例患者,与基线比较随访5年后颈动脉IMT增厚(0.84 mm比0.92 mm,P=0.004)。按rs2071023基因型分组,结果发现CC基因型组颈动脉IMT在随访5年后增厚(0.86 mm比0.97 mm),不同基因型对IMT增厚的贡献,差异具有统计学意义(P=0.012)。结论 PEPCK基因rs2071023位点的CC基因型可能与2型糖尿病患者颈动脉内中膜增厚有关

Follow-up study of the correlation between PEPCK genomic variants and arterial intima-media thickness in patients withtype2 diabetes

Objective To investigate the relationship between rs2071023 variant of phosphoenolp-yruvate carboxykinase (PEPCK) gene and arterial intima-media thickness (IMT) in patients with type 2 diabetes (T2DM).Methods A total of 121 newly diagnosed T2DM patients with normal IMT were enrolled in this study and followed up for 5 years.Genetic polymorphism of rs 2071023 variant of PEPCK gene was determined by PCR-RFLP method.IMT of carotid,femoral artery and iliac artery were determined by Doppler ultrasonography.Results Compared to baseline,carotid IMT was increased significantly after 5 year follow-up (0.84 mm vs.0.92 mm,P =0.004).Further study showed that genotype C/C was associated with carotid IMT (0.97 mm vs.0.86 ram,P = 0.012).Conclusions There has possible association between rs2071023 C/C variant of PEPCK gene and IMT in patients with type 2 diabetes.