Phylogenetic groups and antimicrobial resistance characteristics of Escherichia coli strains isolated from clinical samples in North Iran |
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| Institution: | 1. Department of Microbiology, Islamic Azad University, Lahijan Branch, Iran;2. Department of Microbiology, Zanjan University of Medical Sciences, Zanjan, Iran;3. Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran;4. Department of Microbiology, Guilan University of Medical Sciences, Rasht, Iran;1. Endemic Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt;2. Department of Microbial Biotechnology, Biotechnology Research Institute, National Research Centre, Giza, Egypt;3. Department of Pharmacy, Al-Mustaqbal University College, Babylon, Iraq;4. Clinical Pharmacy unit, Badr University Hospital, Helwan University, Egypt;1. Hepatology and Gastroenterology Department, National Liver Institute, Menoufia University, Egypt;2. Department of Internal Medicine, Salem Medical Center and Center for Alcohol Research and Liver Disease, University of Heidelberg, Germany;3. Endemic Medicine and Hepato-gastroenterology Department, Faculty of Medicine, Cairo University, Cairo, Egypt;4. Gynecology and Obstetrics Department, Faculty of Medicine, Cairo University, Cairo, Egypt;1. Department of General Surgery, Cerrahpasa Medical Faculty, ?stanbul University- Cerrahpasa, ?stanbul, Turkey;2. Department of Pathology, Cerrahpasa Medical Faculty, ?stanbul University- Cerrahpasa, ?stanbul, Turkey;1. Division of Gastroenterology, Bezmialem Vakif University, Istanbul, Turkey;2. Division of Internal Medicine, Bezmialem Vakif University, Istanbul, Turkey;3. Bezmialem Vakif University School of Medicine, Istanbul, Turkey |
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| Abstract: | Background and study aimExtraintestinal pathogenic Escherichia coli (ExPEC) is one of the most common bacterial pathogens, which causes a remarkable amount of morbidity and mortality. This study was designed to determine the antibiotic resistance profiles, phylogenetic groups, and subgroup analyses among the ExPEC strains isolated from hospitalized patients in north Iran.Patients and MethodsThis cross-sectional investigation was conducted at five educational hospitals in Rasht in north Iran. Using standard microbiological tests, 150 E. coli isolates were identified. The antibiotic susceptibility pattern of all isolates was determined using the disk diffusion method. The double disk phenotypic confirmatory test was performed to detect extended-spectrum β-lactamase (ESBL)-producing isolates. A triplex polymerase chain reaction (PCR) was performed to determine the phylogenetic group of each strain.ResultsThe results of antibiogram pattern showed that E. coli isolates were mostly non-susceptible to ampicillin (79.3%), followed by nalidixic acid (75.3%) and cephalothin (70%), whereas nitrofurantoin (94.7%) was the most effective agent, followed by imipenem (92.7%). The rate of ESBL-producing isolates was 53.3% (80/150). Multiplex PCR screening revealed that the most common phylogroup was the B2 group (97 isolates; 64.6%), followed by the D group (34, 22.7%). In contrast, phylogroup analyses showed that B23 (50.7%) and D2 (16.4%) were the most common subgroups.ConclusionsOur findings indicated a considerable rate of antibiotic resistance and ESBL-producing isolates among E. coli strains isolated from clinical samples. Moreover, we reported a tendency that most isolates belonged to the B2 and D phylogroups. As a result, the detection of genotypic identical or similar isolates indicated that these isolates have an endurance capability in the hospital environment and could be transmitted among patients. |
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| Keywords: | Antibiotics resistance Extended-spectrum beta-lactamase Phylogenetic groups |
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