| 苦瓜蛋白通过下调miR-23b-3p促进三磷酸腺苷结合盒转运体A1表达 |
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| 引用本文： | 王艳,费明珠,陈姣姣,王卓,马小峰,杨璐,王佐.苦瓜蛋白通过下调miR-23b-3p促进三磷酸腺苷结合盒转运体A1表达[J].中国动脉硬化杂志,2018,26(4):335-341. |
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| 作者姓名： | 王艳 费明珠 陈姣姣 王卓 马小峰 杨璐 王佐 |
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| 作者单位： | 南华大学附属第一医院病理科;南华大学心血管疾病研究所;邵阳医学高等专科学校附属医院妇产科 |
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| 基金项目： | 国家自然科学基金项目( 81070221)；湖南省卫生计生委科研计划课题(B2016139)；湖南省教育厅课题(16C1392) |
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| 摘 要： | 目的前期工作发现苦瓜蛋白(MD28)可上调THP-1源性泡沫细胞三磷酸腺苷结合盒转运体A1(ABCA1)的表达而减少胞内的脂质蓄积,但其机制不清楚。文章拟从转录后水平分析其作用机制。方法首先用miRNA芯片技术分析经MD28干预后,50 mg/L ox-LDL处理48 h的THP-1源性泡沫细胞miRNA谱中下调1.5倍的micro RNA(miRNA),并与Genecards调控ABCA1基因表达的miRNA比较,找到二者共同miRNA,然后以荧光素酶报告基因系统验证其靶基因关系。qRT-PCR检测ABCA1 m RNA和miR-23b-3p的表达水平,Western blot检测蛋白表达水平,油红O染色测定胞内脂质蓄积。结果 miR-23b-3p是miRNA芯片和Genecards的交集,靶基因验证实验证实ABCA1是miR-23b-3p的靶基因。MD28剂量(0 g/L、0.4 g/L、1.2 g/L、3.6 g/L、5 g/L)和时间(0 h、6 h、12h、24 h、48 h)依赖性地上调ABCA1 m RNA及蛋白的表达水平,以1.2 g/L作用12 h最为显著。ox-LDL上调miR-23b-3p表达且被MD28抑制,MD28能减少胞内脂质蓄积,miR-23b-3p的抑制剂可拮抗MD28对ABCA1表达和胞内脂质蓄积的作用。结论 MD28通过下调miR-23b-3p介导其促进THP-1源性泡沫细胞ABCA1表达、减少胞内脂质蓄积作用。
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| 关 键 词： | 苦瓜蛋白 三磷酸腺苷结合盒转运体A1 hsa-miR-23b-3p 脂质蓄积 |
| 收稿时间： | 2017/12/11 0:00:00 |
| 修稿时间： | 2018/1/9 0:00:00 |
Momordicin promotes adenosine triphosphate binding cassette transporter A1 expression by down-regulating miR-23b-3p |
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| WANG Yan,FEI Ming-Zhu,CHEN Jiao-Jiao,WNAG Zhuo,MA Xiao-Feng,YANG Lu and WNAG Zuo.Momordicin promotes adenosine triphosphate binding cassette transporter A1 expression by down-regulating miR-23b-3p[J].Chinese Journal of Arteriosclerosis,2018,26(4):335-341. |
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| Authors: | WANG Yan FEI Ming-Zhu CHEN Jiao-Jiao WNAG Zhuo MA Xiao-Feng YANG Lu and WNAG Zuo |
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| Institution: | Department of Pathology, the First Affiliated Hospital, ;Institute of Cardiovascular Diseases, University of South China, Hengyang, Hunan 421001,Department of Obstetrics and Gynecology, Affiliated Hospital of Shaoyang Medical College, Shaoyang, Hunan 422000, China,Institute of Cardiovascular Diseases, University of South China, Hengyang, Hunan 421001,Institute of Cardiovascular Diseases, University of South China, Hengyang, Hunan 421001,Institute of Cardiovascular Diseases, University of South China, Hengyang, Hunan 421001,Institute of Cardiovascular Diseases, University of South China, Hengyang, Hunan 421001 and Institute of Cardiovascular Diseases, University of South China, Hengyang, Hunan 421001 |
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| Abstract: | |
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| Keywords: | Momordicin Adenosine triphosphate binding cassette transporter A1 hsa-miR-23b-3p Lipid accumulation |
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