复合膳食纤维对高胆固醇血症大鼠胆固醇代谢的长期作用

健康、断乳SD大鼠 84只 ,经高脂饲料诱导形成高脂血症模型后按体重及血胆固醇均衡的原则分为 7组 ,分别以基础饲料及高脂饲料作为对照组 ,其余 5组饲以不同水平的复合膳食纤维配方B(可溶性纤维 不可溶性纤维 =2 .0 ) ,实验期为 3个月 ,观察配方B对大鼠血浆胆固醇代谢的长期作用。结果表明 :与高脂组相比 ,高胆固醇血症大鼠的血总胆固醇、低密度脂蛋白胆固醇在不同水平的复合膳食纤维配方B组均显著降低 (P <0 .0 5 ) ,而高密度脂蛋白胆固醇水平及高密度脂蛋白胆固醇 低密度脂蛋白胆固醇比值显著升高 (P <0 .0 5 ) ;复合膳食纤维配方B在降低大鼠血总胆固醇浓度的作用上 ,存在明显的剂量反应关系。以上结果提示 :以燕麦麸、沙棘皮、瓜儿豆胶为主要原料研制成的复合膳食纤维确能长期有效地降低血浆胆固醇水平 ,可用于动脉粥样硬化和冠心病的防治。

Long-term Effect of Dietary Fiber Complex on Cholesterol Metabolism in Hypercholesterolemic Rats

Aim To investigate the long term effect of dietary fiber complex (DFC) B at different levels on cholesterol metabolism in hypercholesterolemic rats. Methods 84 healthy SD rats were fed with diet rich in lipids and hypercholesterolemic animal models were established. The rats were randomly divided into 7 groups: hypercholesterolemic control group(HC), 4%DFC group, 8%DFC group, 16%DFC group, 32%DFC group and 64%DFC group. Wheat fiber was used in the hypercholesterolemic control group and rats fed with normal diet were used as control group. Different levels of DFC B were given in the experimental groups. Results The concentrations of plasma total cholesterol (TC),low density lipoprotein cholesterol (LDLC) were all significantly lowered by DFC B at different levels and those of high density lipoprotein cholesterol (HDLC) were significantly elevated compared with hypercholesterolemic control group (P<0.05);Dose response relationship was observed in the effect of DFC on plasma TC. Conclusion DFC composed mainly of Hippophae rhamnoides L bran, oat bran and guar gum can reduce the plasma TC, LDLC and raise HDLC effectively in hypercholesterolemic rats, thus could be used for preventing and therapying atherosclerosis and coronary heart disease.