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实验性家兔缺血预适应减轻心肌细胞凋亡
引用本文:姚震,陈颜芳,冯建章,尹瑞兴,焦解歌,翁阳.实验性家兔缺血预适应减轻心肌细胞凋亡[J].中国动脉硬化杂志,1999,7(1):22-25.
作者姓名:姚震  陈颜芳  冯建章  尹瑞兴  焦解歌  翁阳
作者单位:[1]海南医学院附属医院 [2]广东省心血管病研究所
摘    要:为探讨缺血预适应在预防或减轻心肌损伤和心肌细胞凋亡中的作用,制备了家兔缺血预适应、缺血-再灌注损伤和持续缺血模型,并采用末端脱氧核苷酸转换酶介导的生物素平移缺口末端标记技术检测心肌细胞凋亡情况;同时观察外周血心肌酶、氧自由基等水平。结果发现,再灌注损伤组和持续缺血组血清心肌酶含量明显高于正常组和无显著差异(P〉0.05);同时,前两组的血浆过氧化物歧化酶活性明显降低(P〈0.01),而在正常组和缺

关 键 词:心肌缺血  再灌注损伤  细胞凋亡  预适应

Effect of Experimental Ischemic Preconditioning on Reducing Myocyte Apoptosis in Rabbits
Abstract:Aim To study the role of ischemic preconditioning in reducing myocardial injuries and reducing myocyte apoptosis. Methods The models of ischemic preconditioning (IP), ischemia reperfusion injury (RI) and continuous ischemia (CI) were made with rabbits.The presence of apoptotic myocytes was demonstrated by the method of terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). Also, the activities of plasma superoxide dismutase(SOD) were determined by the method of xanthine oxidase and the contents of serum malonic aldehyde (MDA) were checked by colorimetry. Their serum cardiac enzymes including aspartate transaminase (AST), lactate dehydrogenase(LDH), creatine kinase(CK)and hydroxybutyrate dehydrogenase(HBD) were checked at same time. Results The serum contents of AST, LDH,CK and HBD in RI and CI groups were higher than those in IP and control groups (P< 0.01), which meaned that there was severe damage in myocytes of RI and CI groups. Plasma activities of SOD in RI and CI groups decreased(P<0.01, compared with IP and control groups), but there was no statistic significance between IP and control groups. The contents of serum MDA in RI group was also higher than other groups (P<0.01 or 0.05). The rate of apoptotic myocytes in IP group (24.44±2.96%)was higher than that in control(0.71±0.51%), but was lower than that in CI (29.56±3.08%) and RI groups (43.33±4.92%) significantly (P<0.001). Conclusion This study suggested that IP could prevent myocardial injury induced by ischemia-reperfusion and continuous ischemia , and also reduce the rates of myocyte apoptosis in those situations.
Keywords:Myocardial Ischemia  Reperfusion Injury  Apoptosis  Creatine Kinese  Superoxide Dismutase  Models Cardiouascular  Rabbits  
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