| Notch信号通路介导血小板源生长因子AA诱导的血管平滑肌细胞增殖和迁移 |
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| 引用本文： | 王金祥,周芳,朱光旭,刘菊芬,朱向情,康华莉,庞荣清,黄岚,潘兴华.Notch信号通路介导血小板源生长因子AA诱导的血管平滑肌细胞增殖和迁移[J].中国动脉硬化杂志,2016,24(9):882-888. |
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| 作者姓名： | 王金祥 周芳 朱光旭 刘菊芬 朱向情 康华莉 庞荣清 黄岚 潘兴华 |
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| 作者单位： | 成都军区昆明总医院细胞生物治疗中心 云南省细胞生物医药技术国家地方联合实验室,云南省昆明市 650032;成都军区昆明总医院细胞生物治疗中心 云南省细胞生物医药技术国家地方联合实验室,云南省昆明市 650032;昆明医科大学成都军区昆明总医院临床学院,云南省昆明市 650032;成都军区昆明总医院细胞生物治疗中心 云南省细胞生物医药技术国家地方联合实验室,云南省昆明市 650032;成都军区昆明总医院细胞生物治疗中心 云南省细胞生物医药技术国家地方联合实验室,云南省昆明市 650032;成都军区昆明总医院细胞生物治疗中心 云南省细胞生物医药技术国家地方联合实验室,云南省昆明市 650032;第三军医大学新桥医院全军心血管疾病研究所,重庆市 430037;成都军区昆明总医院细胞生物治疗中心 云南省细胞生物医药技术国家地方联合实验室,云南省昆明市 650032;第三军医大学新桥医院全军心血管疾病研究所,重庆市 430037;成都军区昆明总医院细胞生物治疗中心 云南省细胞生物医药技术国家地方联合实验室,云南省昆明市 650032 |
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| 基金项目： | 国家自然科学基金(81170316)资助 |
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| 摘 要： | 目的探讨Notch信号通路在血小板源生长因子AA(PDGF-AA)诱导血管平滑肌细胞(VSMC)增殖和迁移中的作用及机制。方法脱臼处死1~2月龄雄性SD大鼠,无菌取腹主动脉,剥弃外膜及去除内皮后,贴块法培养VSMC,α-SM-actin染色鉴定。实验分为四组:对照组、γ-secretase抑制剂组(DAPT组)、PDGF-AA组和PDGFAA+γ-secretase抑制剂组(PDGF-AA+DAPT组)。实时荧光定量PCR(Real-time q PCR)检测Notch信号分子mRNA表达;Cell Titer96 Aqueous One Solution试剂盒检测细胞增殖活性;采用细胞划痕实验检测VSMC的迁移能力。结果腹主动脉VSMC表达Notch信号通路Jagged1、Jagged2、Notch1~3等几种主要的配体及受体;PDGF-AA刺激24h和48 h后分别导致Jagged2和Jagged1 mRNA表达显著增强(P0.01,n=4);与0 h相比,PDGF-AA刺激72 h后,Notch1、Notch3 mRNA表达显著增高(P0.01,n=4),而刺激24 h后Notch2 mRNA表达显著降低。PDGF-AA显著促进HES1(P0.05,n=4)、HEY2(P0.05,n=4)和转录因子Rbp-jКmRNA(P0.05,n=4)表达,DAPT抑制PDGF-AA介导的HES1、HEY2 mRNA表达增强(P0.01,n=4),但是进一步促进PDGF-AA诱导的Rbp-jКmRNA表达(P0.05,n=4);PDGF-AA刺激促进VSMC增殖(P0.01,n=5)和减少划痕余留面积(P0.01,n=4),两种效应均可被DAPT显著阻抑(P0.01,n=4)。结论 PDGF-AA调节了VSMC Notch信号分子表达,PDGF-AA部分通过激活Notch信号通路调节VSMC的增殖和迁移。
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| 关 键 词： | Notch 血管平滑肌细胞 血小板源生长因子AA 细胞增殖 细胞迁移 |
| 收稿时间： | 2015/7/3 0:00:00 |
| 修稿时间： | 2015/10/13 0:00:00 |
Notch Signaling Pathway Is Involved in Platelet Derived Growth Factor-AA Induced Vascular Smooth Muscle Cells Proliferation and Migration |
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| WANG Jin-Xiang,ZHOU Fang,ZHU Guang-Xu,LIU Ju-Fen,ZHU Xiang-Qing,KANG Hua-Li,PANG Rong-Qing,HUANG Lan and PAN Xing-Hua.Notch Signaling Pathway Is Involved in Platelet Derived Growth Factor-AA Induced Vascular Smooth Muscle Cells Proliferation and Migration[J].Chinese Journal of Arteriosclerosis,2016,24(9):882-888. |
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| Authors: | WANG Jin-Xiang ZHOU Fang ZHU Guang-Xu LIU Ju-Fen ZHU Xiang-Qing KANG Hua-Li PANG Rong-Qing HUANG Lan and PAN Xing-Hua |
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| Institution: | Cell Biological Therapy Center of Kunming General Hospital, Chengdu Military Region of PLA & Cell Biological Medicine Integrated Engineering Laboratory of State and Region of Yunnan Province, Kunming, Yunnan 650032, China;Cell Biological Therapy Center of Kunming General Hospital, Chengdu Military Region of PLA & Cell Biological Medicine Integrated Engineering Laboratory of State and Region of Yunnan Province, Kunming, Yunnan 650032, China;Clinical College of Medicine,PLA Kunming General Hospital, Kunming Medical University, Kunming,Yunnan 650032, China;Cell Biological Therapy Center of Kunming General Hospital, Chengdu Military Region of PLA & Cell Biological Medicine Integrated Engineering Laboratory of State and Region of Yunnan Province, Kunming, Yunnan 650032, China;Cell Biological Therapy Center of Kunming General Hospital, Chengdu Military Region of PLA & Cell Biological Medicine Integrated Engineering Laboratory of State and Region of Yunnan Province, Kunming, Yunnan 650032, China;Cell Biological Therapy Center of Kunming General Hospital, Chengdu Military Region of PLA & Cell Biological Medicine Integrated Engineering Laboratory of State and Region of Yunnan Province, Kunming, Yunnan 650032, China;Cardiovascular Institute of Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China;Cell Biological Therapy Center of Kunming General Hospital, Chengdu Military Region of PLA & Cell Biological Medicine Integrated Engineering Laboratory of State and Region of Yunnan Province, Kunming, Yunnan 650032, China;Cardiovascular Institute of Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China;Cell Biological Therapy Center of Kunming General Hospital, Chengdu Military Region of PLA & Cell Biological Medicine Integrated Engineering Laboratory of State and Region of Yunnan Province, Kunming, Yunnan 650032, China |
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| Abstract: | |
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| Keywords: | Notch Vascular Smooth Muscle Cell Platelet Derived Growth Factor-AA Cell Proliferation Cell Migration |
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