白细胞介素8－251A/T单核苷酸多态性及其血浆水平与冠心病易感性的关系

目的研究白细胞介素8-251A/T单核苷酸多态性及其血浆水平与冠心病易感性的关系。方法应用聚合酶链反应限制片长多态性检测冠心病组与对照组白细胞介素8-251A/T单核苷酸多态性,酶联免疫吸附法测定白细胞介素8血浆水平。结果白细胞介素8血浆水平在冠心病组显著高于对照组(P<0.05),-251A/T基因型频率、等位基因A和T频率在两组间差异无统计学意义(P>0.05)。但是与其他冠心病患者相比,急性冠状动脉综合征患者AA基因型明显减少(OR=0.43,95%CI为0.2～0.97,P<0.05)。结论白细胞介素8血浆水平与冠状动脉粥样硬化病变有相关性。-251A/T位点单核苷酸多态性与冠心病无相关性,但可影响冠心病的临床表现。AA基因型或许能降低急性冠状动脉综合征的发病风险。

Relationship Between IL-8 -251A/T Single Nucleotide Polymorphism and Plasma Level with Coronary Heart Disease Susceptibility

Aim To study the relationship between plasma level of interleukin-8(IL-8) and the polymorphism of IL-8 single nucleotide with the susceptibility to coronary heart disease(CHD).Methods Polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP) was used to detect genotype of IL-8-251A/T in CHD cases and control subjects.Plasma levels of IL-8 were detected by enzyme-linked immunosorbent assay.Results Compared with that of control subjects,plasma levels of IL-8 of CHD cases were significantly increased(P<0.05).The overall distribution of IL-8-251A/T genotype and allelotype in CHD patients and controls were not significantly different(P>0.05).However,a statistically significant lower frequency of AA genotypes was observed in acute coronary syndrome patients compared to other patients(OR=0.43,95%CI was 0.2~0.97,P=0.04).Conclusions The results suggest that plasma level of IL-8 may co-relate with the susceptibility of CHD,-251A/T single nucleotide polymorphism is not associated with the susceptibility of CHD,but the genetic diversity may influence the clinical manifestation of CHD.The AA genotype may reduce the risk of acute coronary syndrome.