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晚期糖基化终产物对人内皮细胞表达MCP-1和VCAM-1的影响及阿托伐他汀的干预
引用本文:王科峰,徐尚华,许昌声,谢良地.晚期糖基化终产物对人内皮细胞表达MCP-1和VCAM-1的影响及阿托伐他汀的干预[J].中国动脉硬化杂志,2011,19(4).
作者姓名:王科峰  徐尚华  许昌声  谢良地
作者单位:1. 福建医科大学附属南平第一医院心内科,福建省南平市,353000
2. 福建省高血压研究所,福建省福州市,350004
基金项目:福建省科技厅基金项目[(2007)3129]
摘    要:目的观察晚期糖基化终产物对体外培养人脐静脉内皮细胞表达单核细胞趋化蛋白1和血管细胞黏附分子1的影响及阿托伐他汀的干预作用,探讨晚期糖基化终产物诱发动脉粥样硬化形成的机制和阿托伐他汀治疗的机制。方法胶原酶消化法分离获取人脐静脉内皮细胞;倒置相差显微镜形态观察法及免疫细胞化学染色法鉴定人脐静脉内皮细胞;逆转录聚合酶链反应法对单核细胞趋化蛋白1、血管细胞黏附分子1及晚期糖基化终产物受体基因表达进行分析;活性氧检测试剂盒检测细胞内活性氧产生量,倒置荧光显微镜下观察细胞内活性氧荧光强度。结果倒置相差显微镜下可见培养的细胞呈卵圆形或多角形,典型的铺路石样排列,经CD31、CD34染色,细胞浆内有棕黄色颗粒沉着(CD31、CD34阳性)。与空白对照组相比,晚期糖基化终产物(10-4~10-1g/L)呈浓度依赖性增强人内皮细胞单核细胞趋化蛋白1和血管细胞黏附分子1基因表达,10-4g/L晚期糖基化终产物组人内皮细胞单核细胞趋化蛋1和血管细胞黏附分子1基因表达水平开始显著增高(0.26±0.02比0.17±0.04;0.22±0.02比0.08±0.01,P<0.01);与晚期糖基化终产物组相比,阿托伐他汀(0.1、1、10μ...

关 键 词:动脉粥样硬化  晚期糖基化终产物  阿托伐他汀  晚期糖基化终产物受体  活性氧  单核细胞趋化蛋白1  血管细胞黏附分子1  

The Effects of Advanced Glycation End Products on MCP-1 and VCAM-1 Expressions in Human Umbilical Vein Endothelial Cell and the Atorvastatin Intervention Effect
WANG Ke-Feng,XU Shang-Hua,XU Chang-Sheng,XIE Liang-Di.The Effects of Advanced Glycation End Products on MCP-1 and VCAM-1 Expressions in Human Umbilical Vein Endothelial Cell and the Atorvastatin Intervention Effect[J].Chinese Journal of Arteriosclerosis,2011,19(4).
Authors:WANG Ke-Feng  XU Shang-Hua  XU Chang-Sheng  XIE Liang-Di
Institution:WANG Ke-Feng1,XU Shang-Hua1,XU Chang-Sheng2,and XIE Liang-Di2(1.Department of Cardiology,Affiliated Nanping First Hospital of Fujian Medical University,Nanping,Fujian 353000,2.The Hypertension Institute of Fujian Province,Fuzhou,Fujian 350004,China)
Abstract:Aim To investigate the effects of advanced glycation end products(AGE) on the expression of monocyte chemoattractant protein-1(MCP-1) and vascular cell adhesion molecule-1(VCAM-1) in human umbilical vein endothelial cell(HUVEC)and the intervention effect of atorvastatin. Methods Collagenase was used to isolate the endothelial cell from human umbilical vein;HUVEC were identified by immunocellochemistry and morphology;RT-PCR was performed to detect MCP-1,VCAM-1 and recepter for AGE(RAGE) mRNA expression;React...
Keywords:Atherosclerosis  Advanced Glycation end Products  Atorvastatin  Recepter for Advanced Glycation end Products  Reactive Oxygen Species  Monocyte Chemoattractant Protein-1  Vascular Cell Adhesion Molecule-1  
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