昆明白族儿童幽门螺杆菌感染与HLA-DQA1等位基因的相关性

目的:探讨昆明白族儿童HLA-DQA1等位基因位点上是否存在幽门螺杆菌(H pylori)感染的易感基因或抵抗基因,研究H pylori感染的免疫遗传机制．方法:应用聚合酶链反应-序列特异性引物 (PCR-SSP)方法对应用胶体金标疫渗滤法 (DIGFA)和免疫印迹法联合诊断的32例H pylori感染昆明白族儿童(实验组)和34例无 H pylori感染的昆明白族儿童(对照组)进行 HLA-DOA1基因分型检测．结果:实验组HLA-DQA1*010401或*0402基因频率明显低于对照组,两组比较差异存在显著性(0 vs 15％,X2=0,P=0．001,Pc=0．014, OR=1．172,95％CI:1．062-1．294)．结论:HLA-DQA1*010401或*0402基因可能对H pylori感染具有免疲抵抗作用．

Association between HLADQA1 alleles and H pylori infection in Bai-ethnic children from Kunming

AIM: To find the susceptible or resistant genes of H pylori infection in Bai-ethnic children of Kunming, and to explore the immunogenetic mechanism of H pylori infection. METHODS: Polymerase chain reaction-sequence specific primers (PCR-SSP) technique was used to detect the allelic distribution of HLA-DQA1 in Bai-ethnic children with or without H pylori infection (n = 32, 34, respectively) from Kunming. H pylori infection was identified by combination of dot-immunogold filtration assay and immu nobloting technique. RESULTS: The allelic frequency of HLA DQA1 *010401 or *0402 was significantly lower in children with H pylori infection than that in children without H pylori infection (0 vs 15%, x2 = 0, P = 0.001, Pc = 0.014, OR = 1.172, 95%CI: 1.062-1.294). CONCLUSION: HLA-DQA1*010401 or *0402 may be resistant gene of H pylori infection in Bai-ethnic children from Kunming.