白屈菜红碱对肝纤维化大鼠肝脏TGF-β1和α-SMA表达的影响

目的:探讨白屈菜红碱对四氯化碳诱导肝纤维化大鼠肝脏组织转化生长因β1(TGF-β1)和α-平滑肌肌动蛋白(α-SMA)表达的影响.方法:用四氯化碳皮下注射, 同时联合营养控制和饮用100 mL/L乙醇复合法制备SD大鼠肝纤维化模型, 在实验第4周末, 肝纤维化模型建立(2期肝纤维化形成), 然后用低、中、高剂量白屈菜红碱(0.2、0.6、2.0 g/L)治疗, 同时实验设立病理模型组、空白对照和阳性对照(INF-γ)组. 给药8 wk后, 采用免疫组织化学检测各组大鼠肝脏组织TGF-β1和α-SMA的表达.结果:各剂量白屈菜红碱组肝脏TGF-β1和α-SMA表达明显低于病理模型组(TGF-β1:6.08±2.35, 4.31±2.10, 4.7±1.70 vs 9.33±3.08; α-SMA:3.75±1.76, 3.23±1.42, 3.20±1.17 vs 6.67±2.29, 均P <0.01), 而与INF-γ组比较无明显差异(4.23±2.24, 3.38±1.39, 均P >0.05).结论:白屈菜红碱能降低四氯化碳诱导的肝纤维化大鼠模型肝脏组织TGF-β1和α-SMA.

Effects of chelerythine on hepatic TGF-β1 and α-SMA expression in rats with hepatic fibrosis

AIM: To study the effects of chelerythine on TGF-β1 and α-SMA expression of CCl4 -induced hepatic fibrosis in rats. METHODS: Models of hepatic fibrosis were established by hypodermic injection of tetrachloride, in combination with the control of nutrition and the drinking of 100 mL/L alcohol to rats. According to histological sections, hepatic fibrosis in rats emerged at the end of the fourth week. Subsequently different doses of chelerythine were used of hepatic fibrosis in rats. In addition, normal control group, fibrotic model group, γ-interform group in experiment was arranged. At the end of the eighth week, all the rats were executed. Transforming growth factor-β1 (TGF-β1) and α-smooth muscle actin (α-SMA) in liver were examined with the immunohistochemistrical technique. RESULTS: The expression of TGF-β1 and α-SMA in liver of rats in fibrotic model group induced by CCl4 were ameliorated significantly compared with the model group (TGF-β1: 6.08 ±2.35, 4.31 ± 2.10, 4.7 ± 1.70 vs 9.33 ± 3.08; α-SMA:3.75 ± 1.76, 3.23 ± 1.42, 3.20 ± 1.17 vs 6.67 ± 2.29, all P < 0.01). The expression of TGF-β1 and α-SMA in liver was not obviously different between all chelerythine groups and γ-INF group (4.23 ± 2.24, 3.38 ± 1.39, both P > 0.05). CONCLUSION: Chelerythine can decrease the expression of TGF-β1 as well as α-SMA CCl4 -induced hepatic fibrosis in rats.