| 转基因重组质粒ATP4B-SV40T-IRES-GFP的构建及鉴定 |
| |
| 引用本文： | 张隆,赵成根,李大力,王金玉,李琦,范忠泽.转基因重组质粒ATP4B-SV40T-IRES-GFP的构建及鉴定[J].世界华人消化杂志,2012(4):314-318. |
| |
| 作者姓名： | 张隆 赵成根 李大力 王金玉 李琦 范忠泽 |
| |
| 作者单位： | 上海中医药大学附属普陀医院;上海中医药大学中西医结合肿瘤介入研究所;华东师范大学生命医学研究所 |
| |
| 基金项目： | 上海市科委科技发展基金实验动物专项基金资助项目,No.09140903300,No.10140902600;上海市重点学科基金资助项目,No.S30302;上海市教育委员会创新重点项目基金资助项目,No.12ZZ118;上海市卫生局重点基金资助项目,No.2010019~~ |
| |
| 摘 要： | 目的:构建ATP4B-SV40T-IRES-GFP重组质粒并进行鉴定.方法:根据小鼠ATP4B启动子序列设计引物,获得的小鼠ATP4B基因启动子序列,并加入限制性内切酶酶切位点.将其装入载体IRES-GFP,获得重组载体ATP4B-IRES-GFP并进行酶切鉴定.之后将SV40T基因克隆入ATP4B-IRES-GFP载体,并用限制性内切酶PstI和AseⅠ进行验证.结果:将小鼠ATP4B启动子序列插入IRES-GFP载体,经过酶切验证获得正确的ATP4B-IRES-GFP质粒;将SV40T片段插入ATP4B-IRES-GFP,质粒测序及酶切结果验证获得正确的ATP4B-SV40T-IRES-GFP重组质粒.显微注射入小鼠受精卵可获得阳性小鼠.结论:成功构建ATP4B-SV40T-IRES-GFP重组质粒,可用于下一步构建原发性胃癌转基因小鼠.
|
| 关 键 词： | SV40T ATP4B启动子 质粒 转基因小鼠 |
Construction and identification of ATP4B-SV40T-IRES-GFP transgenic vector |
| |
| Long Zhang,Cheng-Gen Zhao,Jin-Yu Wang,Qi Li,Zhong-Ze Fan.Construction and identification of ATP4B-SV40T-IRES-GFP transgenic vector[J].World Chinese Journal of Digestology,2012(4):314-318. |
| |
| Authors: | Long Zhang Cheng-Gen Zhao Jin-Yu Wang Qi Li Zhong-Ze Fan |
| |
| Institution: | ,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Interventional Oncology Institute of Integrative Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China Da-Li Li,Institute of Biomedical Sciences,East China Normal University,Shanghai 200062,China |
| |
| Abstract: | AIM:To construct and identify ATP4B-SV40TIRES-GFP transgenic vector.METHODS:The mouse ATP4B gene promoter was amplified and inserted into IRES-GFP vector after the addition of restriction enzyme sitesto result in ATP4B-IRES-GFP.After restriction enzyme digestion,the SV40T gene sequence was inserted into ATP4B-IRES-GFP.The resultant recombinant plasmid was identified by digestion with restriction enzymes PstI and AseⅠ.RESULTS:The recombinant plasmids ATP4BIRES-GFP and ATP4B-SV40T-IRES-GFP have been successfully constructed and can be used to generate transgenic mice.CONCLUSION:The recombinated plasmid ATP4B-SV40T-IRES-GFP was constructed successfully and it could be used to construct the transgenic mice with primary gastric cancer. |
| |
| Keywords: | SV40T ATP4B Promoter Vector Transgenic mice |
| 本文献已被 CNKI 等数据库收录！ |
|
