同型半胱氨酸水平与冠心病的关系

目的观察同型半胱氨酸(Hey)水平与冠心病(CHD)的关系,并探讨蛋氨酸合成酶还原酶(MTRR)A66G基因多态性、叶酸、维生素B12(VitB12)与Hcy水平及CHD的关系。方法 选择190例经冠状动脉造影证实的CHD患者(CHD组)和100例冠状动脉造影正常者为对照组。应用荧光偏振免疫分析法测定Hey水平,离子捕获分析法测定叶酸水平,微粒酶免疫分析法测定VitB12水平。聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法分析MTRRA66G基因多态性。结果 CHD组血Hcy水平显著高于对照组(15.8±8.8)μmol/L与(12.9±6.3)μmol/L,P=0.002。叶酸、VitB12水平与血Hcy水平呈负相关。叶酸、VitB12与CFD无关。MTRR A66G基因多态性GG纯合子、AG杂合子和AA野生型3种基因型组间血Hcy水平差异无显著性意义。(P=0.908)。MTRR A66G各基因型在CHD组和对照组的分布差异无显著性意义(P=0.198)。结论CHD患者血Hcy水平升高,叶酸、VitB12水平与血Hcy水平呈负相关。MTRR A66G基因多态性与血Hcy水平及与CHD均无关。

The study of association between plasma homocysteine level and coronary heart disease

Objective To study the association between the plasma homocysteine levels and coronary heart disease(CHD) and the associations of methionine synthase recuctase (MTRR) A66G polymorphisms, folate, VitB12 and plasma homocysteine levels with CHD. Methods 190 CHD patients documented by coronary angio-gram and 100 patients with normal angiographic results were included in this study. Plasma homocysteine,folate and VitB12 levels were measured. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to analyse the MTRR A66G genotypes. Results The plasma homocysteine levels were significantly higher in CHD group than in the cont(?)ol group( 16.8±8.8 vs 12.9±6.3μmol/L, P = 0.002). Both the folate and VitB12 levels were negatively correlated to the plasma homocysteine level, but were not correlated to CHD. The differences of the plasma homocysteine levels among the MTRR 66GG, AG and AA genotypes were not statistically significant ( P = 0. 908). There was no significant difference in the prevalence of the MTRR A66G genotypes between CHD group and the control group ( P = 0.198). Conclusion The plasma homocysteine level in coronary heart disease patients was increased. The plasma folate and vitamine12 levels were negatively correlated to plasma homocysteine and MTRR A66G genotypes are not related to plasma homocysteine level and presence of coronary heart disease.