XPA、XPC、XPD、XRCC1基因单核苷酸多态性与急性淋巴细胞白血病易感性的关系

目的 研究中国人群DNA修复基因XPA A23G、XPC C499T和A939C、XPD T751G、XRCC1 G399A和C194T单核苷酸多态性与急性淋巴细胞白血病(ALL)遗传易感性的关系。方法 采用病例-对照研究方法，以Mass-ASSAY平台的基质辅助激光解吸电离飞行时间串联质谱(MALDI-TOF-MS)技术对114例确诊的ALL病例和169例年龄、性别相匹配的正常对照者进行多态性检测，比较不同基因型与ALL风险的关系。结果 与携带XPA 23AA基因型者相比，携带至少1个23G等位基因(即23GG和23AG基因型)的个体ALL风险是其2.02倍(95％CI1.08 ～3.78)，而同时有XPAA23G和XPC C499T 2个位点突变者ALL风险是其5.60倍(95％ CI 1.57～19.90)。XPD T751G、XRCC1 G399A和C194T多态性与ALL易感性之间无显著相关性。结论 XPA A23G和ⅪC C499T 多态性可能与中国人群ALL遗传易感性有关，且存在显著的协同效应。

The relationship between polymorphism of genes XPA, XPC, XPD, XRCC1 and susceptibility to acute lymphoblastic leukemia

Objective To study the relationship between polymorphism of genes XPA, XPC, XPD,XRCC1 and susceptibility to acute lymphoblastic leukemia (ALL) in a Chinese population.Methods Polymorphism were determined by a case-control study through matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry method of Mass-ASSAY platform in 114 confirmed ALL cases and 169 age- and sex- matched controls, so as to compare the relationship between differert genotypes and ALL risk.Results Multivariate logistic regression analysis revealed that individuals carrying at least one 23G variant allele(AG/GG genotypes) had a significantly increased risk for ALL (adjusted OR 2.02; 95％ CI 1.08-3.78) compared with the wild-type genotype (23 AA), and evidence that positive interactions between the polymorphisms in XPC C499T and XPA A23G might occur.Furthermore, individuals with both putative risk genotypes had a significantly higher risk (adjusted OR 5.60; 95％ CI 1.57-19.90), compared with those with both wild-genotypes. By contrast, no significant association was observed between the XPD T751G, XRCC1 G399A, C194T pelymorphism and ALL risk.Conclusions XPA A23G and XPC C499T polymorphism may contribute to the risk of developing ALL.There are significant combinations between XPC C499T and XPA A23G.