白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

目的 研究我同湖北汉族人群IL-10及TNF的基因多态性与胃十二指肠疾病及幽门螺杆菌(Hp)感染的关系.方法 采用病例财照研究和聚合酶链反应.限制性片段长度多态性(PCR-RFLP)方法检测605例胃十二指肠疾病患者(包括196例慢性胃炎、189例胃十二指肠溃疡及220例胃癌患者)和624例健康对照者中IL-10基因启动子区3个位点(IL-10-1082/-819/-592)的等位基因型和TNFα-308、LTα(淋巴毒素α,亦称TNFβ)Nco Ⅰ、AspH Ⅰ双等位基因型分布;用ELISA法检测血清中Hp-IgG及cagA-IgG抗体水平.结果 (1)IL-10-1082 AG+GG基因型在胃癌组、慢性胃炎组及胃十二指肠溃疡组(非胃癌组)、健康对照组分布频率分别为20.0%、7.5%、6.0%和5.0%,IL-10-1082 AG+GG基因型分布在非胃痛组及健康对照组之间差异无统计学意义(P>0.05),而胃癌组高于非胃癌组(P<0.05)及健康对照组(P<0.05),其差异均有统计学意义.胃癌组中IL-10-592及-819两个位点与非胃癌组及健康对照组比较,基因型分布差异无统计学意义(P>0.05).IL-10-819位点与IL-10-592位点基因型分布频率一致.(2)胃癌组与其余3组比较,IL-10-1082 AG+GG基因型且Hp阳性的分布频率的差异有统计学意义(P<0.05).(3)LTα Nco Ⅰ AG基因型在Hp阳性胃癌患者中(66.7%)高于Hp阳性的健康对照组(47.8%),差异有统计学意义(P<0.05),该基因型与其他胃十二指肠疾病尤相关性.TNFα-308、LTα AspH Ⅰ与Hp感染及胃十二指肠疾病亦无相关性.结论 (1)IL-10-1082 AG+GG基因型与湖北地区汉族人群胃癌发生有相关性.(2)IL-10-1082 AG+GG基因型和LTα Nco Ⅰ AG基因型与湖北汉族人群Hp阳性胃癌有相关性.

The relationship among IL-10, TNF gene polymorphisms, Helicobacter pylori infection and gastroduodenal diseases in Hubei Han ethnic

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.