HGF及其受体c-met在肝衰竭与部分肝切除模型中表达差异性研究

目的研究HGF及其受体c-met在肝衰竭和部分肝切除两种动物模型肝组织的表达差异。方法分别采用D-GalN/LPS腹腔注射和70%肝脏外科切除术建立肝衰竭和部分肝切除模型,同时以健康大鼠作为对照组。采用ELISA法检测血清HGF水平,采用Real-time PCR和Western-blot法分别检测肝组织c-met基因和蛋白的表达水平。结果与对照组比,部分肝切除模型大鼠血清HGF及其受体c-met表达水平均有明显升高（P〈0.01）,而在肝衰竭模型大鼠血清HGF水平虽有升高,但c-met蛋白表达水平却明显降低（P〈0.01）。结论肝细胞c-met表达水平的降低是D-GalN/LPS诱导肝衰竭大鼠肝细胞再生障碍的主要原因。

Hepatic growth foctor and its receptor c-met expressions in rat models with liver failure and partial hepatectomy

Objective To study hepatic growth factor（HGF）and c-met expressions in rat models with liver failure and partial hepatectomy.Methods Liver failure induced by intraperitoneal injection of LPS/D-GalN and by 70% partial hepatectomy was established,and healthy rats served as controls.The level of serum HGF was detected by ELISA assay and c-met gene expression were detected by real-time PCR and Western-bloting.Results Compared with the control group,the serum HGF level and its receptor c-met were significantly elevated in rats with partial hepatectomy,however,the serum HGF level increased with c-met expression significantly reduced in rats with liver failure.Conclusion The compromised hepatic c-met expression might be essential for defects in HGF-mediated liver regeneration in rats with liver failure.