| 两个标准化cNDA差减文库的构建 |
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| 引用本文： | 田海生,马磊,李秀兰,朱昌亮.两个标准化cNDA差减文库的构建[J].中国血吸虫病防治杂志,2001,13(2):75-78. |
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| 作者姓名： | 田海生 马磊 李秀兰 朱昌亮 |
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| 作者单位： | 南京医科大学寄生虫学教研室,南京,210029 |
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| 基金项目： | 本研究受国家自然科学基金资助(39970666) |
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| 摘 要： | 目的 构建2个标准化cDNA差减文库，方法 以淡色库蚊对溴氰菊酯抗药性品系为Tester、敏感性品系为Driver，进行正向抑制性差减杂交；以敏感吕系为Tester、抗性品系为Driver，进行反向抑制性差减杂交，分别将获取的2组抑制性差减杂交产物克隆入质粒表达载体（Pin-Point^TMXa-1TVector)，并以PCR扩增鉴定插入片段。结果 获得2个标准化cDAN差减文库，其中抗性相关基因（抗性品系高表达或特异表达）文库含523个重组子，敏感相关基因（敏感品系高表达或特异表达）文库含286个重组子，插入片段大小平均约360bp。结论 所建的2个差减文库适合做进一步研究。
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| 关 键 词： | 淡色库蚊 抑制性差减杂交 cDNA差减文库 标准化 |
| 文章编号： | 1005-6661(2001)02-0075-04 |
| 修稿时间： | 2000年7月10日 |
CONSTRUCTION OF 2 NORMALIZED cDNA SUBTRACTED LIBRARIES |
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| Tian Haisheng,Ma Lei,Li Xiulan,Zhu Changliang.CONSTRUCTION OF 2 NORMALIZED cDNA SUBTRACTED LIBRARIES[J].Chinese Journal of Schistosomiasis Control,2001,13(2):75-78. |
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| Authors: | Tian Haisheng Ma Lei Li Xiulan Zhu Changliang |
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| Institution: | Tian Haisheng,Ma Lei,Li Xiulan,Zhu Changliang Department of Parasitology,Nanjing Medical University,Nanjing 210029 |
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| Abstract: | Objective To construct 2 normalized cDNA subtracted libraries. Methods By suppression subtractive hybridization, differentially expressed genes ofCulex pipiens pallensbetween resistant and susceptible strains were got and cloned into PinPoint plasmid vector. ]Results Two normalized cDNA subtracted libraries were obtained including 523 and 286 recombinants respectively. The length of fragments inserted was average 360 bp.Conclusion The 2 constructing subtracted cDNA libraries were suitable for further study. |
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| Keywords: | Cules pipiens pallens Suppression subtractive hybridization (SSH) Subtracted cDNA library |
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